Monitoring structure development in milk acidification using diffuse reflectance profiles

Jacob Lercke Skytte, Ulf Andersen, Flemming Møller, Otto Højager Attermann Nielsen, Jens Michael Carstensen, Anders Lindbjerg Dahl, Rasmus Larsen

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    The structure of dairy products is important for the consumer, and milk acidification plays a central role for structural development. To ensure the best possible consumer experience, it is important that a product’s structural properties are stable. Therefore process and quality control tools are needed so that the production can be carried out consistently, regardless of day-to-day
    variations in the raw materials.
    Casein micelles aggregate during milk acidification, which leads to formation of a gel network. This change of structure is important for the development of a range of dairy products. It is therefore essential to monitor these structural changes and a variety of methods have been proposed to continuously follow this coagulation of milk [1]. Especially non-invasive methods for in situ production line application have been of interest.
    We propose a method for analyzing structural changes in milk based on hyper-spectral light scattering. Our approach is motivated by Carstensen and Møller [2]. They demonstrated the correlation between diffuse reflectance profiles and rheology of a milk sample during acidification. In this work we employ a super-continuum laser light source coupled with an acousto-optical tuneable filter to illuminate the sample. The generated beam is spectrally narrow and can be tuned in the spectral range from 450-1050 nm. This system is described in detail in [3]. It is a research platform, which is constantly developed and adjusted according to research
    needs. Besides providing a non-invasive method, the system also has potential as a design platform for creating specialized and cost-efficient vision systems.
    Our preliminary results are highly encouraging and show a clear relation between rheology and diffuse reflectance. A factorial experiment studying the effects of the content of fat, protein, and temperature in the acidification process is conducted. The purpose of the experiment is to investigate how the change of these parameters affects the diffuse reflectance properties as well as to demonstrate the relation between the optical parameters and structure formation in milk acidification. These measurements are compared to conventional methods such as pH, oscillatory rheology, confocal laser scanning microscopy, and sensory data.
    Original languageEnglish
    Title of host publicationProceedings of the 14th Food Colloids Conference 2012
    PublisherUniversity of Copenhagen
    Publication date2012
    Publication statusPublished - 2012
    EventFood Colloids 2012: Creation and Breakdown of Structure - Copenhagen, Denmark
    Duration: 16 Apr 201218 Apr 2012


    ConferenceFood Colloids 2012
    Internet address

    Cite this

    Skytte, J. L., Andersen, U., Møller, F., Nielsen, O. H. A., Carstensen, J. M., Dahl, A. L., & Larsen, R. (2012). Monitoring structure development in milk acidification using diffuse reflectance profiles. In Proceedings of the 14th Food Colloids Conference 2012 University of Copenhagen.