Monitoring of Saccharomyces cerevisiae cell proliferation on thiol-modified planar gold microelectrodes using impedance spectroscopy

Arto Heiskanen, Christer F Spegel, Natalie Kostesha, Tautgirdas Ruzgas, Jenny Emnéus

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance (R-ct) to the redox process of [Fe(CN)6](3-14-) showed a linear relationship to the number of cells even beyond the monolayer coverage. R,, showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R,, showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated that real-time monitoring of S. cerevisiae proliferation, with frequency-normalized imaginary admittance (real capacitance) as the indicator, was possible using a miniaturized culture system, ECIS Cultureware, with integrated planar cysteamine-modified Au microelectrodes. A monolayer coverage was reached after 20-28 h of cultivation, observed as an similar to 15% decrease in the real capacitance of the system.
    Original languageEnglish
    JournalLangmuir
    Volume24
    Issue number16
    Pages (from-to)9066-9073
    ISSN0743-7463
    DOIs
    Publication statusPublished - 2008

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