Molecular techniques and data integration: investigating distribution system coliform events

S. J. Payne, M. C. Besner, J. Lavoie, C. Krentz, Lisbeth Truelstrup Hansen, M. Friedman, M. Friedman, M. Prevost, G. A. Gagnon

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A retrospective analysis of coliform events in a drinking water distribution system was conducted. A total of 13 coliform isolates with specific geographic/sampling records were analysed using microbial source tracking (MST) to establish genetic (based on DNA) and phenotypic (based on substrate utilization) relatedness between isolates, and a data integration approach (geo-spatial and event analysis) to identify potential causes for their presence. Phenotypic analysis, using the API (R) 20E system, identified two major genera: Enterobacter and Citrobacter, showing a high degree of phenotypic similarity within each species. However genotypic analysis (using pulsed field gel electrophoresis) of the Enterobacter and Citrobacter isolates demonstrated a wide genetic diversity within each species. The data integration approach revealed the probable causes for coliform events were the installation of new mains (8 samples) and rehabilitation of existing main (1 sample). The data integration and MST approach (genotyping) suggested that these coliform events were a result of sporadic intrusion from maintenance events and not chronic biofilm re-growth. The combination of MST and the data integration approach offers utilities a potentially powerful tool for the examination and determination of coliform occurrences in a distribution system.
Original languageEnglish
JournalJOURNAL OF WATER SUPPLY RESEARCH AND TECHNOLOGY-AQUA
Volume59
Issue number5
Pages (from-to)298-311
ISSN0003-7214
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Water Science and Technology
  • Health, Toxicology and Mutagenesis
  • Environmental Engineering
  • Coliform
  • Data integration
  • Distribution system
  • Drinking water
  • Microbial source tracking
  • Pulsed field gel electrophoresis
  • Coliform data
  • Distribution systems
  • Biomarkers
  • Electrophoresis
  • Gels
  • Integration
  • Potable water
  • Water distribution systems
  • Water supply
  • Data handling
  • biofilm
  • coliform bacterium
  • data management
  • drinking water
  • electrokinesis
  • genetic analysis
  • genotype
  • phenotype
  • pollutant source
  • water supply
  • Citrobacter
  • Enterobacter
  • ENGINEERING,
  • WATER
  • NEGATIVE ESCHERICHIA-COLI
  • SOURCE TRACKING
  • DRINKING-WATER
  • IDENTIFICATION
  • PATTERNS
  • BACTERIA
  • DISEASE
  • SAMPLES
  • FIELD
  • coliform
  • data integration
  • distribution system
  • microbial source tracking
  • pulsed field gel electrophoresis
  • drinking water distribution system
  • genetic diversity
  • genetic relation
  • phenotypic relation
  • sporadic intrusion
  • substrate utilization
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Enterobacter genus contaminant Citrobacter genus contaminant
  • DNA
  • 03509, Genetics - Population genetics
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 31000, Physiology and biochemistry of bacteria
  • 37014, Public health - Sewage disposal and sanitary measures
  • 37015, Public health - Air, water and soil pollution
  • Population Studies
  • API 20E system field equipment
  • event analysis applied and field techniques
  • genotypic analysis laboratory techniques, genetic techniques
  • geospatial analysis applied and field techniques
  • microbial source tracking applied and field techniques
  • phenotypic analysis laboratory techniques, genetic techniques
  • pulsed field gel electrophoresis electrophoretic techniques, laboratory techniques
  • Methods and Techniques
  • Pollution Assessment Control and Management
  • Population Genetics
  • Sanitation
  • CITROBACTER
  • DRINKING WATER
  • ENTEROBACTER
  • FOOD SAFETY BEVERAGES
  • POTABLE WATER
  • WATER SUPPLIES
  • Alcoholic and non-alcoholic beverages
  • Water

Cite this

Payne, S. J., Besner, M. C., Lavoie, J., Krentz, C., Hansen, L. T., Friedman, M., Friedman, M., Prevost, M., & Gagnon, G. A. (2010). Molecular techniques and data integration: investigating distribution system coliform events. JOURNAL OF WATER SUPPLY RESEARCH AND TECHNOLOGY-AQUA, 59(5), 298-311. https://doi.org/10.2166/aqua.2010.026