Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status

Daniel Pergament Persson, Thomas C. de Bang, Pai R. Pedas, Umit Baris Kutman, Ismail Cakmak, Birgit Andersen, Christine Finnie, Jan K. Schjoerring, Søren Husted

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    Abstract

    Low concentration of zinc (Zn) in the endosperm of cereals is a major factor contributing to Zn deficiency in human populations. We have investigated how combined Zn and nitrogen (N) fertilization affects the speciation and localization of Zn in durum wheat (Triticum durum). Zn-binding proteins were analysed with liquid chromatography ICP-MS and Orbitrap MS2, respectively. Laser ablation ICP-MS with simultaneous Zn, sulphur (S) and phosphorus (P) detection was used for bioimaging of Zn and its potential ligands. Increasing the Zn and N supply had a major impact on the Zn concentration in the endosperm, reaching concentrations higher than current breeding targets. The S concentration also increased, but S was only partly co-localized with Zn. The mutual Zn and S enrichment was reflected in substantially more Zn bound to small cysteine-rich proteins (apparent size 10-30 kDa), whereas the response of larger proteins (apparent size > 50 kDa) was only modest. Most of the Zn-responsive proteins were associated with redox- and stress-related processes. This study offers a methodological platform to deepen the understanding of processes behind endosperm Zn enrichment. Novel information is provided on how the localization and speciation of Zn is modified during Zn biofortification of grains.
    Original languageEnglish
    JournalNew Phytologist
    Volume211
    Issue number4
    Pages (from-to)1255-1265
    Number of pages11
    ISSN0028-646X
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Durum wheat
    • Endosperm
    • Inductively coupled plasma-mass spectrometry (ICP-MS)
    • Laser ablation (LA)
    • Speciation
    • Zinc (Zn) localization
    • Zinc (Zn)-binding proteins

    Cite this

    Persson, D. P., de Bang, T. C., Pedas, P. R., Kutman, U. B., Cakmak, I., Andersen, B., Finnie, C., Schjoerring, J. K., & Husted, S. (2016). Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status. New Phytologist, 211(4), 1255-1265. https://doi.org/10.1111/nph.13989