Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis

Stephanie Walter; Sajid Ali; Eric Kemen; Kumarse Nazari; Bochra A. Bahri; Jérôme Enjalbert; Jens G. Hansen; James K.M. Brown; Thomas Sicheritz-Pontén; Jonathan Jones; Claude de Vallavieille-Pope; Mogens S. Hovmøller; Annemarie Fejer Justesen

doi:10.1002/ece3.2069

Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis

Stephanie Walter, Sajid Ali, Eric Kemen, Kumarse Nazari, Bochra A. Bahri, Jérôme Enjalbert, Jens G. Hansen, James K.M. Brown, Thomas Sicheritz-Pontén, Jonathan Jones, Claude de Vallavieille-Pope, Mogens S. Hovmøller, Annemarie Fejer Justesen

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Abstract

Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.

Original language	English
Journal	Ecology and Evolution
Volume	6
Issue number	9
Pages (from-to)	2790-2804
Number of pages	15
ISSN	2045-7758
DOIs	https://doi.org/10.1002/ece3.2069
Publication status	Published - 2016

Bibliographical note

© 2016 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

Aggressive strain
PCR-based markers
Plant pathogen
Wheat yellow rust/stripe rust

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Walter, S., Ali, S., Kemen, E., Nazari, K., Bahri, B. A., Enjalbert, J., Hansen, J. G., Brown, J. K. M., Sicheritz-Pontén, T., Jones, J., de Vallavieille-Pope, C., Hovmøller, M. S., & Justesen, A. F. (2016). Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecology and Evolution, 6(9), 2790-2804. https://doi.org/10.1002/ece3.2069

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abstract = "Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.",

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Walter, S, Ali, S, Kemen, E, Nazari, K, Bahri, BA, Enjalbert, J, Hansen, JG, Brown, JKM, Sicheritz-Pontén, T, Jones, J, de Vallavieille-Pope, C, Hovmøller, MS & Justesen, AF 2016, 'Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis', Ecology and Evolution, vol. 6, no. 9, pp. 2790-2804. https://doi.org/10.1002/ece3.2069

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T1 - Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis

AU - Walter, Stephanie

AU - Ali, Sajid

AU - Kemen, Eric

AU - Nazari, Kumarse

AU - Bahri, Bochra A.

AU - Enjalbert, Jérôme

AU - Hansen, Jens G.

AU - Brown, James K.M.

AU - Sicheritz-Pontén, Thomas

AU - Jones, Jonathan

AU - de Vallavieille-Pope, Claude

AU - Hovmøller, Mogens S.

AU - Justesen, Annemarie Fejer

N1 - © 2016 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2016

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N2 - Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.

AB - Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.

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KW - PCR-based markers

KW - Plant pathogen

KW - Wheat yellow rust/stripe rust

U2 - 10.1002/ece3.2069

DO - 10.1002/ece3.2069

M3 - Journal article

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SN - 2045-7758

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JO - Ecology and Evolution

JF - Ecology and Evolution

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