TY - JOUR
T1 - Molecular Fingerprints of the Human Fecal Microbiota From 9 to 18 Months Old and the Effect of Fish Oil Supplementation
AU - Andersen, Anders Daniel
AU - Mølbak, Lars
AU - Michaelsen, Kim Fleischer
AU - Lauritzen, Lotte
PY - 2011
Y1 - 2011
N2 - Objectives: The aim of this study was to monitor changes in the fecal
microbiota from 9 to 18 months and to investigate the effect of increasing
dietary n-3 polyunsaturated fatty acids on the fecal microbiota.
Patients and Methods: In a double-blind controlled trial with random
allocation to daily supplementation with 5mL of fish oil (FO) or sunflower
oil (SO) from 9 to 18 months of age, stool samples were collected from 132
healthy Danish infants. Molecular fingerprints of the bacterial DNA were
obtained by terminal restriction fragment length polymorphism (T-RFLP).
Results: The T-RFLP profiles indicated that a few T-RFs became dominant
with age (bp100 and 102, both presumed to be Bacteroidetes) concomitantly
with an overall increase in the microbial diversity (P¼0.04). Breast-feeding
influenced both the T-RFLP profiles at 9 months and the changes from 9 to
18 months, and breast-feeding cessation during the trial modified the
response to the dietary oils. In the FO group, the increase in bp102 was
significantly reduced among children weaned before compared with those
weaned during the trial (P¼0.027), whereas the increase in bp100 was
reduced in the preweaned children of the SO group relative to those weaned
during the trial (P¼0.004). This was supported by intervention group
differences in the changes in bp102 and bp100 among the earlier weaned
children (P¼0.06 and P¼0.09, respectively).
Conclusions: Cessation of breast-feeding played a dominant role relative to
developmental changes in the fecal microbiota from 9 to 18 months. FO
compared with SO supplementation affected changes in large bacterial
groups, but only among children who had stopped breast-feeding before
9 months of age.
AB - Objectives: The aim of this study was to monitor changes in the fecal
microbiota from 9 to 18 months and to investigate the effect of increasing
dietary n-3 polyunsaturated fatty acids on the fecal microbiota.
Patients and Methods: In a double-blind controlled trial with random
allocation to daily supplementation with 5mL of fish oil (FO) or sunflower
oil (SO) from 9 to 18 months of age, stool samples were collected from 132
healthy Danish infants. Molecular fingerprints of the bacterial DNA were
obtained by terminal restriction fragment length polymorphism (T-RFLP).
Results: The T-RFLP profiles indicated that a few T-RFs became dominant
with age (bp100 and 102, both presumed to be Bacteroidetes) concomitantly
with an overall increase in the microbial diversity (P¼0.04). Breast-feeding
influenced both the T-RFLP profiles at 9 months and the changes from 9 to
18 months, and breast-feeding cessation during the trial modified the
response to the dietary oils. In the FO group, the increase in bp102 was
significantly reduced among children weaned before compared with those
weaned during the trial (P¼0.027), whereas the increase in bp100 was
reduced in the preweaned children of the SO group relative to those weaned
during the trial (P¼0.004). This was supported by intervention group
differences in the changes in bp102 and bp100 among the earlier weaned
children (P¼0.06 and P¼0.09, respectively).
Conclusions: Cessation of breast-feeding played a dominant role relative to
developmental changes in the fecal microbiota from 9 to 18 months. FO
compared with SO supplementation affected changes in large bacterial
groups, but only among children who had stopped breast-feeding before
9 months of age.
KW - Infancy
KW - Gut bacteria
KW - n-3 polyunsaturated fatty acids
U2 - 10.1097/MPG.0b013e31821d298f
DO - 10.1097/MPG.0b013e31821d298f
M3 - Journal article
C2 - 21865979
VL - 53
SP - 303
EP - 309
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 3
ER -