Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy†

A M Zolini, J Block, Maria Belen Rabaglino, P Tribulo, M Hoelker, G Rincon, J J Bromfield, Peter Juul Hansen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence to establish and maintain gestation. Embryos produced with X-sorted sperm were cultured from day 5 to day 7 in serum-free medium containing 10 ng/ml recombinant bovine CSF2 or vehicle. The CSF2 was administered because this molecule can increase blastocyst competence for survival after embryo transfer. Blastocysts were harvested on day 7 of culture and manually bisected. One demi-embryo from a single blastocyst was transferred into a synchronized recipient and the other half was used for RNA-seq analysis. Using P  2-fold or 
Original languageEnglish
JournalBiology of Reproduction
Volume102
Issue number2
Pages (from-to)292–305
ISSN0006-3363
DOIs
Publication statusPublished - 2020

Fingerprint Dive into the research topics of 'Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy†'. Together they form a unique fingerprint.

Cite this