Abstract
An endo-1,4-beta-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temperature of 40 A degrees C and pH of 5.5. Using oat-spelt xylan, the determined K (m), V (max), and k (cat)/K (m) values were 0.243 mg ml(-1), 4,530 U mg(-1) protein, and 7,640 ml s(-1) mg(-1), respectively. A homology model and sequence analysis of XylCg, along with the biochemical properties, confirmed that XylCg belongs to the GH11 family. Rice straw pretreated with XylCg showed 30 % higher conversion yield than the rice straw pretreated with a commercial xylanase. Although xylanases have been characterized from fungal and bacterial sources, C. globosum XylCg is distinguished from other xylanases by its high catalytic efficiency and its effectiveness in the pretreatment of lignocellulosic biomass.
| Original language | English |
|---|---|
| Journal | Applied Microbiology and Biotechnology |
| Volume | 97 |
| Issue number | 16 |
| Pages (from-to) | 7205-7214 |
| ISSN | 0175-7598 |
| DOIs | |
| Publication status | Published - 2013 |
| Externally published | Yes |
Keywords
- Avena sativa
- Biomass
- Chaetomium
- Cloning, Molecular
- Endo-1,4-beta Xylanases
- Enzyme Stability
- Escherichia coli
- Gene Expression
- Hydrogen-Ion Concentration
- Kinetics
- Lignin
- Models, Molecular
- Oryza sativa
- Plant Stems
- Protein Conformation
- Recombinant Proteins
- Temperature
- Xylans
- 11132-73-3 lignocellulose
- 9005-53-2 Lignin
- EC 3.2.1.8 Endo-1,4-beta Xylanases
- BIOTECHNOLOGY
- TRICHODERMA-REESEI
- XYLANASE GENE
- ESCHERICHIA-COLI
- EXPRESSION
- PURIFICATION
- HYDROLYSIS
- HYDROLASES
- Chaetomium globosum
- Endo-1,4-beta-xylanase
- Homology modeling
- Pretreatment
- Saccharification
- crop yield
- lignocellulosic biomass
- rice straw
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species
- Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Chaetomium globosum species
- Monocotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Monocots, Plants, Spermatophytes, Vascular Plants) - Gramineae [25305] rice common
- Chaetomium globosum xylcg gene [Ascomycetes] Chaetomium globosum endo-1,4-beta-xylanase gene
- GH11 endoxylanase
- oat-spelt xylan
- xylanase 37278-89-0 EC 3.2.1.32
- 03502, Genetics - General
- 03504, Genetics - Plant
- 10802, Enzymes - General and comparative studies: coenzymes
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- 39008, Food microbiology - General and miscellaneous
- 51518, Plant physiology - Enzymes
- 52502, Agronomy - Miscellaneous and mixed crops
- Agriculture
- Biochemistry and Molecular Biophysics
- enzymatic biomass pretreatment laboratory techniques
- molecular characterization laboratory techniques, genetic techniques
- molecular cloning laboratory techniques, genetic techniques
- Agronomy
- Bioprocess Engineering
- Enzymology
- Methods and Techniques
- Molecular Genetics
- Genes
- Cloning
- endo 1,4 beta xylanase
- xylan endo 1,3 beta xylosidase
- biomass
- clonal growth
- enzyme
- enzyme activity
- fungus
- gene
- homology
- straw
- article
- chaetomium globosum
- controlled study
- enzyme analysis
- enzyme purification
- molecular cloning
- nonhuman
- oat
- pH
- protein expression
- rice
- sequence analysis
- Bacteria (microorganisms)
- Triticum aestivum subsp. spelta
- Biochemical properties
- Catalytic efficiencies
- Enzymatic pretreatment
- Lignocellulosic biomass
- Pre-Treatment
- Xylanases
- Endo-1,4-β-xylanase
- BIOMASS
- CHAETOMIUM
- FERMENTATION PRODUCTS
- FUNGI
- GENES
- GENETICS
- GLYCOSIDASES
- XYLAN DEGRADING ENZYMES
- Biotechnology
- Enzyme systems
- MOLECULAR cloning
- HASH(0x4a175c0)