TY - JOUR
T1 - Molecular characterisation of Staphylococcus aureus from some artisanal Brazilian dairies
AU - Alves, Virgínia Farias
AU - Niño-Arias, Fabian Camilo
AU - Pitondo-Silva, André
AU - Araújo Frazilio, Diego de
AU - Oliveira Gonçalves, Larissa de
AU - Toubas, Luíza Chaul
AU - Sapateiro Torres, Ieda Maria
AU - Oxaran, Virginie
AU - Dittmann, Karen Kiesbye
AU - De Martinis, Elaine Cristina Pereira
PY - 2018
Y1 - 2018
N2 - The clonal relationships and antimicrobial resistance of Staphylococcus aureus isolates from three fresh Minas cheese production lines were investigated. Putative S. aureus were confirmed by 16S rRNA gene sequencing and were characterised by multilocus sequence typing (MLST) and antimicrobial susceptibility. Overall, 33 out of 64 samples were contaminated with S. aureus (51.6%). The contamination rates of the dairies were 60.8%, 21% and 68.2%. Thirty representative isolates were selected to be typed by MLST and five sequence types (ST) were found (ST1, 97, 126, 3531), including the new ST 3816. The clonal complex 1 (CC1) was dominant. Resistance to commonly used antimicrobials was observed but only one strain was resistant to methicillin. This methicillin resistant S. aureus strain belonged to the CC126 that is commonly associated with mastitis in ruminants. The dissemination of zoonotic bacteria through the food chain is not a frequent event but represents a concern to public health.
AB - The clonal relationships and antimicrobial resistance of Staphylococcus aureus isolates from three fresh Minas cheese production lines were investigated. Putative S. aureus were confirmed by 16S rRNA gene sequencing and were characterised by multilocus sequence typing (MLST) and antimicrobial susceptibility. Overall, 33 out of 64 samples were contaminated with S. aureus (51.6%). The contamination rates of the dairies were 60.8%, 21% and 68.2%. Thirty representative isolates were selected to be typed by MLST and five sequence types (ST) were found (ST1, 97, 126, 3531), including the new ST 3816. The clonal complex 1 (CC1) was dominant. Resistance to commonly used antimicrobials was observed but only one strain was resistant to methicillin. This methicillin resistant S. aureus strain belonged to the CC126 that is commonly associated with mastitis in ruminants. The dissemination of zoonotic bacteria through the food chain is not a frequent event but represents a concern to public health.
U2 - 10.1016/j.idairyj.2018.06.008
DO - 10.1016/j.idairyj.2018.06.008
M3 - Journal article
SN - 0958-6946
VL - 85
SP - 247
EP - 253
JO - International Dairy Journal
JF - International Dairy Journal
ER -
