Molecular beacon – tool for real time studying gene activity in stem cells.

Mirolyuba Ilieva, Martin Dufva

    Research output: Contribution to conferencePosterResearchpeer-review

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    Abstract

    Cells respond to their internal genetic programs and external stimuli by modulating the synthesis of specific mRNAs. Direct observation of mRNA expression in living cells can provide valuable information with regards to understanding fundamental processes such cell differentiation, regeneration and cancerogenesis. Molecular beacon technology is based on fluorescence resonance energy transfer (FRET) and the complementary pairing principles. These fluorescent molecular probes are highly specific and sensitive and are one important tool in in vitro diagnostics. Here molecular beacons are used to follow expression of stem cell markers and neural markers in live differentiating cells.
    Original languageEnglish
    Publication date2012
    Number of pages1
    Publication statusPublished - 2012
    EventEMBL conference: Stem Cells in Cancer and Regenerative Medicine - Heidelberg, Germany
    Duration: 29 Aug 20121 Sep 2012

    Conference

    ConferenceEMBL conference: Stem Cells in Cancer and Regenerative Medicine
    CountryGermany
    CityHeidelberg
    Period29/08/201201/09/2012

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