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  • Author: Ma, Xiao Qing

    Aarhus University, Denmark

  • Author: Jepsen, Morten Leth

    Aarhus University

  • Author: Ivarsen, Anne Kathrine R.

    Aarhus University, Denmark

  • Author: Knudsen, Birgitta R.

    Aarhus University, Denmark

  • Author: Ho, Yi Ping

    Chinese University of Hong Kong, China

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Multicellular spheroids have garnered significant attention as an in vitro three-dimensional cancer model which can mimick the in vivo microenvironmental features. While microfluidics generated double emulsions have become a potential method to generate spheroids, challenges remain on the tedious procedures. Enabled by a novel 'airway resistance' based selective surface treatment, this study presents an easy and facile generation of double emulsions for the initiation and cultivation of multicellular spheroids in a scaffold-free format. Combining with our previously developed DNA nanosensors, intestinal spheroids produced in the double emulsions have shown an elevated activities of an essential DNA modifying enzyme, the topoisomerase I. The observed molecular and functional characteristics of spheroids produced in double emulsions are similar to the counterparts produced by the commercially available ultra-low attachment plates. However, the double emulsions excel for their improved uniformity, and the consistency of the results obtained by subsequent analysis of the spheroids. The presented technique is expected to ease the burden of producing spheroids and to promote the spheroids model for cancer or stem cell study.
Original languageEnglish
Article number095014
JournalJournal of Micromechanics and Microengineering
Number of pages9
Publication statusPublished - 2017
Externally publishedYes
CitationsWeb of Science® Times Cited: No match on DOI

ID: 140592262