TY - JOUR
T1 - Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus
AU - Stenfeldt, Anna Carolina
AU - Heegaard, Peter M. H.
AU - Stockmarr, Anders
AU - Belsham, Graham
PY - 2012
Y1 - 2012
N2 - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples
from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at
different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour
necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain
reaction. Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during
the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA
compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples
collected during both acute and late (>28 days post infection) phases of infection. There were also significantly
higher levels of TNF-a mRNA expressed in samples derived from animals that were identified
subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference
in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of
FMDV.
AB - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples
from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at
different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour
necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain
reaction. Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during
the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA
compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples
collected during both acute and late (>28 days post infection) phases of infection. There were also significantly
higher levels of TNF-a mRNA expressed in samples derived from animals that were identified
subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference
in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of
FMDV.
KW - Pharyngeal epithelium
KW - Cytokine mRNA
KW - Foot-and-mouth disease
U2 - 10.1016/j.jcpa.2011.06.005
DO - 10.1016/j.jcpa.2011.06.005
M3 - Journal article
SN - 0021-9975
VL - 146
SP - 243
EP - 252
JO - Journal of Comparative Pathology
JF - Journal of Comparative Pathology
IS - 2-3
ER -