Modular microfluidic system as a model of cystic fibrosis airways

Maciej Skolimowski, Martin Weiss Nielsen , Fabien Abeille, Peder Skafte-Pedersen, David Sabourin, A. Fercher, D. Papkovsky, Søren Molin, Rafael J. Taboryski, Claus Sternberg, Martin Dufva, Oliver Geschke, Jenny Emnéus

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    Abstract

    A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.
    Original languageEnglish
    JournalBiomicrofluidics
    Volume6
    Issue number3
    Pages (from-to)034109
    ISSN1932-1058
    DOIs
    Publication statusPublished - 2012

    Bibliographical note

    © 2012 American Institute of Physics

    Keywords

    • Antibiotics
    • Bacteria
    • Cell culture
    • Oxygen

    Cite this

    Skolimowski, M., Weiss Nielsen , M., Abeille, F., Skafte-Pedersen, P., Sabourin, D., Fercher, A., ... Emnéus, J. (2012). Modular microfluidic system as a model of cystic fibrosis airways. Biomicrofluidics, 6(3), 034109. https://doi.org/10.1063/1.4742911
    Skolimowski, Maciej ; Weiss Nielsen , Martin ; Abeille, Fabien ; Skafte-Pedersen, Peder ; Sabourin, David ; Fercher, A. ; Papkovsky, D. ; Molin, Søren ; Taboryski, Rafael J. ; Sternberg, Claus ; Dufva, Martin ; Geschke, Oliver ; Emnéus, Jenny. / Modular microfluidic system as a model of cystic fibrosis airways. In: Biomicrofluidics. 2012 ; Vol. 6, No. 3. pp. 034109.
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    title = "Modular microfluidic system as a model of cystic fibrosis airways",
    abstract = "A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.",
    keywords = "Antibiotics, Bacteria, Cell culture, Oxygen",
    author = "Maciej Skolimowski and {Weiss Nielsen}, Martin and Fabien Abeille and Peder Skafte-Pedersen and David Sabourin and A. Fercher and D. Papkovsky and S{\o}ren Molin and Taboryski, {Rafael J.} and Claus Sternberg and Martin Dufva and Oliver Geschke and Jenny Emn{\'e}us",
    note = "{\circledC} 2012 American Institute of Physics",
    year = "2012",
    doi = "10.1063/1.4742911",
    language = "English",
    volume = "6",
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    Skolimowski, M, Weiss Nielsen , M, Abeille, F, Skafte-Pedersen, P, Sabourin, D, Fercher, A, Papkovsky, D, Molin, S, Taboryski, RJ, Sternberg, C, Dufva, M, Geschke, O & Emnéus, J 2012, 'Modular microfluidic system as a model of cystic fibrosis airways', Biomicrofluidics, vol. 6, no. 3, pp. 034109. https://doi.org/10.1063/1.4742911

    Modular microfluidic system as a model of cystic fibrosis airways. / Skolimowski, Maciej; Weiss Nielsen , Martin ; Abeille, Fabien; Skafte-Pedersen, Peder; Sabourin, David; Fercher, A.; Papkovsky, D.; Molin, Søren; Taboryski, Rafael J.; Sternberg, Claus; Dufva, Martin; Geschke, Oliver; Emnéus, Jenny.

    In: Biomicrofluidics, Vol. 6, No. 3, 2012, p. 034109.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - Modular microfluidic system as a model of cystic fibrosis airways

    AU - Skolimowski, Maciej

    AU - Weiss Nielsen , Martin

    AU - Abeille, Fabien

    AU - Skafte-Pedersen, Peder

    AU - Sabourin, David

    AU - Fercher, A.

    AU - Papkovsky, D.

    AU - Molin, Søren

    AU - Taboryski, Rafael J.

    AU - Sternberg, Claus

    AU - Dufva, Martin

    AU - Geschke, Oliver

    AU - Emnéus, Jenny

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    AB - A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.

    KW - Antibiotics

    KW - Bacteria

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    Skolimowski M, Weiss Nielsen M, Abeille F, Skafte-Pedersen P, Sabourin D, Fercher A et al. Modular microfluidic system as a model of cystic fibrosis airways. Biomicrofluidics. 2012;6(3):034109. https://doi.org/10.1063/1.4742911