Abstract
| Original language | English |
|---|---|
| Journal | Biomicrofluidics |
| Volume | 6 |
| Issue number | 3 |
| Pages (from-to) | 034109 |
| ISSN | 1932-1058 |
| DOIs | |
| Publication status | Published - 2012 |
Bibliographical note
© 2012 American Institute of PhysicsKeywords
- Antibiotics
- Bacteria
- Cell culture
- Oxygen
Cite this
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Modular microfluidic system as a model of cystic fibrosis airways. / Skolimowski, Maciej; Weiss Nielsen , Martin ; Abeille, Fabien; Skafte-Pedersen, Peder; Sabourin, David; Fercher, A.; Papkovsky, D.; Molin, Søren; Taboryski, Rafael J.; Sternberg, Claus; Dufva, Martin; Geschke, Oliver; Emnéus, Jenny.
In: Biomicrofluidics, Vol. 6, No. 3, 2012, p. 034109.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Modular microfluidic system as a model of cystic fibrosis airways
AU - Skolimowski, Maciej
AU - Weiss Nielsen , Martin
AU - Abeille, Fabien
AU - Skafte-Pedersen, Peder
AU - Sabourin, David
AU - Fercher, A.
AU - Papkovsky, D.
AU - Molin, Søren
AU - Taboryski, Rafael J.
AU - Sternberg, Claus
AU - Dufva, Martin
AU - Geschke, Oliver
AU - Emnéus, Jenny
N1 - © 2012 American Institute of Physics
PY - 2012
Y1 - 2012
N2 - A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.
AB - A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers. We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment. This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.
KW - Antibiotics
KW - Bacteria
KW - Cell culture
KW - Oxygen
U2 - 10.1063/1.4742911
DO - 10.1063/1.4742911
M3 - Journal article
VL - 6
SP - 034109
JO - Biomicrofluidics
JF - Biomicrofluidics
SN - 1932-1058
IS - 3
ER -