Modelling the effects of dietary methionine level and form on postprandial plasma essential amino acid profiles in rainbow trout (Oncorhynchus mykiss)

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Abstract

Aquafeed formulation is susceptible to affect amino acid (AA) availability for metabolic functions. Statistical models were applied to quantify the effect of dietary methionine level (from 6.01 to 16.17 g kg 1 dry matter) and form (free, coated or bound) on postprandial concentrations of plasma essential amino acid (EAA) in rainbow trout. Twelve diets were formulated with pea and soya protein concentrate or fish meal as the main protein ingredients and were supplemented or not with increasing amount of either crystalline or agar-coated methionine. Fish were acclimatized to one of the 12 diets for 6 weeks before postprandial plasma sampling (six sampling points up to 36 h, seven fish each time), further analysed for EAA content. Using generalized additive models, we show that (i) dietary methionine level and form explained 74% postprandial methionine plasma variations and that (ii) the methionine dietary form and plasma concentrations significantly affected the plasma concentrations of the other EAAs. Finally, linear model revealed a positive relationship (R2 > 0.9) between plasma concentrations of the three branched-chain AAs under the present experimental conditions. The results obtained add new information on the dietary effects on EAAs in the plasma availability and the interactions between them
Original languageEnglish
JournalAquaculture Nutrition
Volume22
Issue number6
Pages (from-to)1185–1201
ISSN1353-5773
DOIs
Publication statusPublished - 2016

Cite this

@article{c2dd65b197cf403388a5adfc711d7877,
title = "Modelling the effects of dietary methionine level and form on postprandial plasma essential amino acid profiles in rainbow trout (Oncorhynchus mykiss)",
abstract = "Aquafeed formulation is susceptible to affect amino acid (AA) availability for metabolic functions. Statistical models were applied to quantify the effect of dietary methionine level (from 6.01 to 16.17 g kg 1 dry matter) and form (free, coated or bound) on postprandial concentrations of plasma essential amino acid (EAA) in rainbow trout. Twelve diets were formulated with pea and soya protein concentrate or fish meal as the main protein ingredients and were supplemented or not with increasing amount of either crystalline or agar-coated methionine. Fish were acclimatized to one of the 12 diets for 6 weeks before postprandial plasma sampling (six sampling points up to 36 h, seven fish each time), further analysed for EAA content. Using generalized additive models, we show that (i) dietary methionine level and form explained 74{\%} postprandial methionine plasma variations and that (ii) the methionine dietary form and plasma concentrations significantly affected the plasma concentrations of the other EAAs. Finally, linear model revealed a positive relationship (R2 > 0.9) between plasma concentrations of the three branched-chain AAs under the present experimental conditions. The results obtained add new information on the dietary effects on EAAs in the plasma availability and the interactions between them",
author = "Marine Rolland and Feekings, {Jordan P.} and Dalsgaard, {Anne Johanne Tang} and J. Holm and Skov, {Peter Vilhelm}",
year = "2016",
doi = "10.1111/anu.12325",
language = "English",
volume = "22",
pages = "1185–1201",
journal = "Aquaculture Nutrition",
issn = "1353-5773",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Modelling the effects of dietary methionine level and form on postprandial plasma essential amino acid profiles in rainbow trout (Oncorhynchus mykiss)

AU - Rolland, Marine

AU - Feekings, Jordan P.

AU - Dalsgaard, Anne Johanne Tang

AU - Holm, J.

AU - Skov, Peter Vilhelm

PY - 2016

Y1 - 2016

N2 - Aquafeed formulation is susceptible to affect amino acid (AA) availability for metabolic functions. Statistical models were applied to quantify the effect of dietary methionine level (from 6.01 to 16.17 g kg 1 dry matter) and form (free, coated or bound) on postprandial concentrations of plasma essential amino acid (EAA) in rainbow trout. Twelve diets were formulated with pea and soya protein concentrate or fish meal as the main protein ingredients and were supplemented or not with increasing amount of either crystalline or agar-coated methionine. Fish were acclimatized to one of the 12 diets for 6 weeks before postprandial plasma sampling (six sampling points up to 36 h, seven fish each time), further analysed for EAA content. Using generalized additive models, we show that (i) dietary methionine level and form explained 74% postprandial methionine plasma variations and that (ii) the methionine dietary form and plasma concentrations significantly affected the plasma concentrations of the other EAAs. Finally, linear model revealed a positive relationship (R2 > 0.9) between plasma concentrations of the three branched-chain AAs under the present experimental conditions. The results obtained add new information on the dietary effects on EAAs in the plasma availability and the interactions between them

AB - Aquafeed formulation is susceptible to affect amino acid (AA) availability for metabolic functions. Statistical models were applied to quantify the effect of dietary methionine level (from 6.01 to 16.17 g kg 1 dry matter) and form (free, coated or bound) on postprandial concentrations of plasma essential amino acid (EAA) in rainbow trout. Twelve diets were formulated with pea and soya protein concentrate or fish meal as the main protein ingredients and were supplemented or not with increasing amount of either crystalline or agar-coated methionine. Fish were acclimatized to one of the 12 diets for 6 weeks before postprandial plasma sampling (six sampling points up to 36 h, seven fish each time), further analysed for EAA content. Using generalized additive models, we show that (i) dietary methionine level and form explained 74% postprandial methionine plasma variations and that (ii) the methionine dietary form and plasma concentrations significantly affected the plasma concentrations of the other EAAs. Finally, linear model revealed a positive relationship (R2 > 0.9) between plasma concentrations of the three branched-chain AAs under the present experimental conditions. The results obtained add new information on the dietary effects on EAAs in the plasma availability and the interactions between them

U2 - 10.1111/anu.12325

DO - 10.1111/anu.12325

M3 - Journal article

VL - 22

SP - 1185

EP - 1201

JO - Aquaculture Nutrition

JF - Aquaculture Nutrition

SN - 1353-5773

IS - 6

ER -