Thiocyanate (SCN−)-based autotrophic denitrification (AD) has recently been demonstrated as a promising technology that could be integrated with anaerobic ammonium oxidation (Anammox) to achieve simultaneous removal of nitrogen and SCN−. However, there is still a lack of a complete SCN−-based AD model, and the potential microbial competition/synergy between AD bacteria and Anammox bacteria under different operating conditions remains unknown, which significantly hinders the possible application of coupling SCN−-based AD with Anammox. To this end, a complete SCN−-based AD model was firstly developed and reliably calibrated/validated using experimental datasets. The obtained SCN−-based AD model was then integrated with the well-established Anammox model and satisfactorily verified with experimental data from a system coupling AD with Anammox. The integrated model was lastly applied to investigate the impacts of influent NH4 +-N/NO2 −-N ratio and SCN− concentration on the steady-state microbial composition as well as the removal of nitrogen and SCN−. The results showed that the NH4 +-N/NO2 −-N ratio in the presence of a certain SCN− level should be controlled at a proper value so that the maximum synergy between AD bacteria and Anammox bacteria could be achieved while their competition for NO2 − would be minimized. For the simultaneous maximum removal (>95%) of nitrogen and SCN−, there existed a negative relationship between the influent SCN− concentration and the optimal NH4 +-N/NO2 −-N ratio needed. High-level (>95%) simultaneous removal of nitrogen and thiocyanate could be achieved through combining thiocyanate-based autotrophic denitrification and Anammox.
- Anaerobic ammonium oxidation
- Microbial competition
- Thiocyanate-based autotrophic denitrification