Mobilization transfer of the pUB110 plasmid between gram-positive bacteria

E. V. Kozlova, Rustam Aminov, A. M. Boronin

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The three factor crosses between the donor strain Bacillus subtilis 168 harbouring the plasmid pUB102-4, Bacillus thuringiensis strain carrying the mobilizing plasmid pAM beta 1 and recipient strain Lactobacillus fermenti were conducted in order to elaborate the optimal conditions of the plasmid pUB102-4 mobilization for transfer into gram-positive microorganisms and to elucidate the possible expression of endogluconase genes in a lactobacillus strain. The Lactobacillus fermenti transconjugants carrying the pUB102-4 plasmid were obtained in the three factor reciprocal crosses with the streptococcus recipient strain and Bacillus subtilis recipients. The presence of the plasmids in transconjugants was confirmed by colony hybridization with the [32P]-labelled plasmid DNA and KMC-ase activity in transconjugant cells. The proposed system of crosses using the high copy number plasmid derivatives of pUB110 mobilized with high frequency by the pAM beta 1 plasmid demonstrates the possibility to increase the circle of gram-positive host bacteria avoiding time and labour consuming operations.
Original languageEnglish
JournalMolekulyarnaya Genetika, Mikrobiologiya i Virusologiya
Issue number9
Pages (from-to)15-17
ISSN0208-0613
Publication statusPublished - 1991
Externally publishedYes

Fingerprint Dive into the research topics of 'Mobilization transfer of the pUB110 plasmid between gram-positive bacteria'. Together they form a unique fingerprint.

Cite this