Mixtures of environmentally relevant endocrine disrupting chemicals affect mammary gland development in female and male rats

Karen Riiber Mandrup, Hanna Katarina Lilith Johansson, Julie Boberg, Anne S. Pedersen, Mette Sidsel Mortensen, Jennifer Solgaard Jørgensen, Anne Marie Vinggaard, Ulla Hass

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Estrogenic chemicals are able to alter mammary gland development in female rodents, but little is known on the effects of anti-androgens and mixtures of endocrine disrupting chemicals (EDCs) with dissimilar modes of action. Pregnant rat dams were exposed during gestation and lactation to mixtures of environmentally relevant EDCs with estrogenic, anti-androgenic or dissimilar modes of action (TotalMix) of 100-, 200- or 450-fold high end human intake estimates. Mammary glands of prepubertal and adult female and male offspring were examined. Oestrogens increased mammary outgrowth in prepubertal females and the mRNA level of matrix metalloproteinase-3, which may be a potential biomarker for increased outgrowth. Mixtures of EDCs gave rise to ductal hyperplasia in adult males. Adult female mammary glands of the TotalMix group showed morphological changes possibly reflecting increased prolactin levels. In conclusion both estrogenic and anti-androgenic chemicals given during foetal life and lactation affected mammary glands in the offspring.
Original languageEnglish
JournalReproductive Toxicology
Volume54
Pages (from-to)47-57
ISSN0890-6238
DOIs
Publication statusPublished - 2015

Keywords

  • Endocrine disrupter
  • Mammary gland
  • Mix
  • Oestrogens
  • Anti-androgens
  • Whole mount
  • Histology
  • Mmp

Cite this

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title = "Mixtures of environmentally relevant endocrine disrupting chemicals affect mammary gland development in female and male rats",
abstract = "Estrogenic chemicals are able to alter mammary gland development in female rodents, but little is known on the effects of anti-androgens and mixtures of endocrine disrupting chemicals (EDCs) with dissimilar modes of action. Pregnant rat dams were exposed during gestation and lactation to mixtures of environmentally relevant EDCs with estrogenic, anti-androgenic or dissimilar modes of action (TotalMix) of 100-, 200- or 450-fold high end human intake estimates. Mammary glands of prepubertal and adult female and male offspring were examined. Oestrogens increased mammary outgrowth in prepubertal females and the mRNA level of matrix metalloproteinase-3, which may be a potential biomarker for increased outgrowth. Mixtures of EDCs gave rise to ductal hyperplasia in adult males. Adult female mammary glands of the TotalMix group showed morphological changes possibly reflecting increased prolactin levels. In conclusion both estrogenic and anti-androgenic chemicals given during foetal life and lactation affected mammary glands in the offspring.",
keywords = "Endocrine disrupter, Mammary gland, Mix, Oestrogens, Anti-androgens, Whole mount, Histology, Mmp",
author = "Mandrup, {Karen Riiber} and Johansson, {Hanna Katarina Lilith} and Julie Boberg and Pedersen, {Anne S.} and Mortensen, {Mette Sidsel} and J{\o}rgensen, {Jennifer Solgaard} and Vinggaard, {Anne Marie} and Ulla Hass",
year = "2015",
doi = "10.1016/j.reprotox.2014.09.016",
language = "English",
volume = "54",
pages = "47--57",
journal = "Reproductive Toxicology",
issn = "0890-6238",
publisher = "Elsevier",

}

Mixtures of environmentally relevant endocrine disrupting chemicals affect mammary gland development in female and male rats. / Mandrup, Karen Riiber; Johansson, Hanna Katarina Lilith; Boberg, Julie; Pedersen, Anne S.; Mortensen, Mette Sidsel; Jørgensen, Jennifer Solgaard; Vinggaard, Anne Marie; Hass, Ulla.

In: Reproductive Toxicology, Vol. 54, 2015, p. 47-57.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Mixtures of environmentally relevant endocrine disrupting chemicals affect mammary gland development in female and male rats

AU - Mandrup, Karen Riiber

AU - Johansson, Hanna Katarina Lilith

AU - Boberg, Julie

AU - Pedersen, Anne S.

AU - Mortensen, Mette Sidsel

AU - Jørgensen, Jennifer Solgaard

AU - Vinggaard, Anne Marie

AU - Hass, Ulla

PY - 2015

Y1 - 2015

N2 - Estrogenic chemicals are able to alter mammary gland development in female rodents, but little is known on the effects of anti-androgens and mixtures of endocrine disrupting chemicals (EDCs) with dissimilar modes of action. Pregnant rat dams were exposed during gestation and lactation to mixtures of environmentally relevant EDCs with estrogenic, anti-androgenic or dissimilar modes of action (TotalMix) of 100-, 200- or 450-fold high end human intake estimates. Mammary glands of prepubertal and adult female and male offspring were examined. Oestrogens increased mammary outgrowth in prepubertal females and the mRNA level of matrix metalloproteinase-3, which may be a potential biomarker for increased outgrowth. Mixtures of EDCs gave rise to ductal hyperplasia in adult males. Adult female mammary glands of the TotalMix group showed morphological changes possibly reflecting increased prolactin levels. In conclusion both estrogenic and anti-androgenic chemicals given during foetal life and lactation affected mammary glands in the offspring.

AB - Estrogenic chemicals are able to alter mammary gland development in female rodents, but little is known on the effects of anti-androgens and mixtures of endocrine disrupting chemicals (EDCs) with dissimilar modes of action. Pregnant rat dams were exposed during gestation and lactation to mixtures of environmentally relevant EDCs with estrogenic, anti-androgenic or dissimilar modes of action (TotalMix) of 100-, 200- or 450-fold high end human intake estimates. Mammary glands of prepubertal and adult female and male offspring were examined. Oestrogens increased mammary outgrowth in prepubertal females and the mRNA level of matrix metalloproteinase-3, which may be a potential biomarker for increased outgrowth. Mixtures of EDCs gave rise to ductal hyperplasia in adult males. Adult female mammary glands of the TotalMix group showed morphological changes possibly reflecting increased prolactin levels. In conclusion both estrogenic and anti-androgenic chemicals given during foetal life and lactation affected mammary glands in the offspring.

KW - Endocrine disrupter

KW - Mammary gland

KW - Mix

KW - Oestrogens

KW - Anti-androgens

KW - Whole mount

KW - Histology

KW - Mmp

U2 - 10.1016/j.reprotox.2014.09.016

DO - 10.1016/j.reprotox.2014.09.016

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C2 - 25305543

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JO - Reproductive Toxicology

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