Mixture toxicity effects and uptake of titanium dioxide (TiO2) nanoparticles and 3,3″,4,4″-tetrachlorobiphenyl (PCB77) in juvenile brown trout following co-exposure via the diet

Tobias Lammel*, Britt Wassmur, Aiga Mackevica, Chang-Er L. Chen, Joachim Sturve

*Corresponding author for this work

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Abstract

Titanium dioxide nanoparticles (n-TiO2) are among the man-made nanomaterials that are predicted to be found at high concentrations in the aquatic environment. There, they likely co-exist with other chemical pollutants. Thus, n-TiO2 and other chemical pollutants can be taken up together or accumulate independently from each other in prey organisms of fish. This can lead to dietary exposure of fish to n-TiO2-chemical pollutant mixtures. In this study, we examine if simultaneous dietary exposure to n-TiO2 and 3,3’,4,4’-Tetrachlorobiphenyl (PCB77) –used as a model compound for persistent organic pollutants with dioxin-like properties– can influence the uptake and toxicological response elicited by the respective other substance. Juvenile brown trout (Salmo trutta) were fed custom-made food pellets containing n-TiO2, PCB77 or n-TiO2+PCB77 mixtures for 15 days. Ti and PCB77 concentrations in the liver were measured by ICP-MS and GC–MS, respectively. Besides, n-TiO2 uptake was assessed using TEM. Combination effects on endpoints specific for PCB77 (i.e., cytochrome P450 1A (CYP1A) induction) and endpoints shared by both PCB77 and n-TiO2 (i.e., oxidative stress-related parameters) were measured in intestine and liver using RT-qPCR and enzyme activity assays. The results show that genes encoding for proteins/enzymes essential for tight junction function (zo-1) and ROS elimination (sod-1) were significantly upregulated in the intestine of fish exposed to n-TiO2 and PCB77 mixtures, but not in the single-substance treatments. Besides, n-TiO2 had a potentiating effect on PCB77-induced CYP1A and glutathione reductase (GR) expression/enzyme activity in the liver. This study shows that simultaneous dietary exposure to nanomaterials and traditional environmental pollutants might result in effects that are larger than observed for the substances alone, but that understanding the mechanistic basis of such effects remains challenging.
Original languageEnglish
Article number105195
JournalAquatic Toxicology
Volume213
Number of pages13
ISSN0166-445X
DOIs
Publication statusPublished - 2019

Keywords

  • Nanomaterial
  • Metal oxide
  • Fish
  • Accumulation
  • Co-contaminant
  • Persistent organic pollutant

Cite this

@article{79f59522e26d41648f5dee18710f03c4,
title = "Mixture toxicity effects and uptake of titanium dioxide (TiO2) nanoparticles and 3,3″,4,4″-tetrachlorobiphenyl (PCB77) in juvenile brown trout following co-exposure via the diet",
abstract = "Titanium dioxide nanoparticles (n-TiO2) are among the man-made nanomaterials that are predicted to be found at high concentrations in the aquatic environment. There, they likely co-exist with other chemical pollutants. Thus, n-TiO2 and other chemical pollutants can be taken up together or accumulate independently from each other in prey organisms of fish. This can lead to dietary exposure of fish to n-TiO2-chemical pollutant mixtures. In this study, we examine if simultaneous dietary exposure to n-TiO2 and 3,3’,4,4’-Tetrachlorobiphenyl (PCB77) –used as a model compound for persistent organic pollutants with dioxin-like properties– can influence the uptake and toxicological response elicited by the respective other substance. Juvenile brown trout (Salmo trutta) were fed custom-made food pellets containing n-TiO2, PCB77 or n-TiO2+PCB77 mixtures for 15 days. Ti and PCB77 concentrations in the liver were measured by ICP-MS and GC–MS, respectively. Besides, n-TiO2 uptake was assessed using TEM. Combination effects on endpoints specific for PCB77 (i.e., cytochrome P450 1A (CYP1A) induction) and endpoints shared by both PCB77 and n-TiO2 (i.e., oxidative stress-related parameters) were measured in intestine and liver using RT-qPCR and enzyme activity assays. The results show that genes encoding for proteins/enzymes essential for tight junction function (zo-1) and ROS elimination (sod-1) were significantly upregulated in the intestine of fish exposed to n-TiO2 and PCB77 mixtures, but not in the single-substance treatments. Besides, n-TiO2 had a potentiating effect on PCB77-induced CYP1A and glutathione reductase (GR) expression/enzyme activity in the liver. This study shows that simultaneous dietary exposure to nanomaterials and traditional environmental pollutants might result in effects that are larger than observed for the substances alone, but that understanding the mechanistic basis of such effects remains challenging.",
keywords = "Nanomaterial, Metal oxide, Fish, Accumulation, Co-contaminant, Persistent organic pollutant",
author = "Tobias Lammel and Britt Wassmur and Aiga Mackevica and Chen, {Chang-Er L.} and Joachim Sturve",
year = "2019",
doi = "10.1016/j.aquatox.2019.04.021",
language = "English",
volume = "213",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",

}

Mixture toxicity effects and uptake of titanium dioxide (TiO2) nanoparticles and 3,3″,4,4″-tetrachlorobiphenyl (PCB77) in juvenile brown trout following co-exposure via the diet. / Lammel, Tobias; Wassmur, Britt; Mackevica, Aiga; Chen, Chang-Er L.; Sturve, Joachim.

In: Aquatic Toxicology, Vol. 213, 105195, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Mixture toxicity effects and uptake of titanium dioxide (TiO2) nanoparticles and 3,3″,4,4″-tetrachlorobiphenyl (PCB77) in juvenile brown trout following co-exposure via the diet

AU - Lammel, Tobias

AU - Wassmur, Britt

AU - Mackevica, Aiga

AU - Chen, Chang-Er L.

AU - Sturve, Joachim

PY - 2019

Y1 - 2019

N2 - Titanium dioxide nanoparticles (n-TiO2) are among the man-made nanomaterials that are predicted to be found at high concentrations in the aquatic environment. There, they likely co-exist with other chemical pollutants. Thus, n-TiO2 and other chemical pollutants can be taken up together or accumulate independently from each other in prey organisms of fish. This can lead to dietary exposure of fish to n-TiO2-chemical pollutant mixtures. In this study, we examine if simultaneous dietary exposure to n-TiO2 and 3,3’,4,4’-Tetrachlorobiphenyl (PCB77) –used as a model compound for persistent organic pollutants with dioxin-like properties– can influence the uptake and toxicological response elicited by the respective other substance. Juvenile brown trout (Salmo trutta) were fed custom-made food pellets containing n-TiO2, PCB77 or n-TiO2+PCB77 mixtures for 15 days. Ti and PCB77 concentrations in the liver were measured by ICP-MS and GC–MS, respectively. Besides, n-TiO2 uptake was assessed using TEM. Combination effects on endpoints specific for PCB77 (i.e., cytochrome P450 1A (CYP1A) induction) and endpoints shared by both PCB77 and n-TiO2 (i.e., oxidative stress-related parameters) were measured in intestine and liver using RT-qPCR and enzyme activity assays. The results show that genes encoding for proteins/enzymes essential for tight junction function (zo-1) and ROS elimination (sod-1) were significantly upregulated in the intestine of fish exposed to n-TiO2 and PCB77 mixtures, but not in the single-substance treatments. Besides, n-TiO2 had a potentiating effect on PCB77-induced CYP1A and glutathione reductase (GR) expression/enzyme activity in the liver. This study shows that simultaneous dietary exposure to nanomaterials and traditional environmental pollutants might result in effects that are larger than observed for the substances alone, but that understanding the mechanistic basis of such effects remains challenging.

AB - Titanium dioxide nanoparticles (n-TiO2) are among the man-made nanomaterials that are predicted to be found at high concentrations in the aquatic environment. There, they likely co-exist with other chemical pollutants. Thus, n-TiO2 and other chemical pollutants can be taken up together or accumulate independently from each other in prey organisms of fish. This can lead to dietary exposure of fish to n-TiO2-chemical pollutant mixtures. In this study, we examine if simultaneous dietary exposure to n-TiO2 and 3,3’,4,4’-Tetrachlorobiphenyl (PCB77) –used as a model compound for persistent organic pollutants with dioxin-like properties– can influence the uptake and toxicological response elicited by the respective other substance. Juvenile brown trout (Salmo trutta) were fed custom-made food pellets containing n-TiO2, PCB77 or n-TiO2+PCB77 mixtures for 15 days. Ti and PCB77 concentrations in the liver were measured by ICP-MS and GC–MS, respectively. Besides, n-TiO2 uptake was assessed using TEM. Combination effects on endpoints specific for PCB77 (i.e., cytochrome P450 1A (CYP1A) induction) and endpoints shared by both PCB77 and n-TiO2 (i.e., oxidative stress-related parameters) were measured in intestine and liver using RT-qPCR and enzyme activity assays. The results show that genes encoding for proteins/enzymes essential for tight junction function (zo-1) and ROS elimination (sod-1) were significantly upregulated in the intestine of fish exposed to n-TiO2 and PCB77 mixtures, but not in the single-substance treatments. Besides, n-TiO2 had a potentiating effect on PCB77-induced CYP1A and glutathione reductase (GR) expression/enzyme activity in the liver. This study shows that simultaneous dietary exposure to nanomaterials and traditional environmental pollutants might result in effects that are larger than observed for the substances alone, but that understanding the mechanistic basis of such effects remains challenging.

KW - Nanomaterial

KW - Metal oxide

KW - Fish

KW - Accumulation

KW - Co-contaminant

KW - Persistent organic pollutant

U2 - 10.1016/j.aquatox.2019.04.021

DO - 10.1016/j.aquatox.2019.04.021

M3 - Journal article

C2 - 31203167

VL - 213

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

M1 - 105195

ER -