Minimal requirements for exponential growth of Lactococcus lactis

Peter Ruhdal Jensen, Karin Hammer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h-1. With 19 amino acids added, the growth rate increased to mu = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
    Original languageEnglish
    JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
    Volume59
    Issue number12
    Pages (from-to)4363-4366
    Number of pages4
    ISSN0099-2240
    Publication statusPublished - 1993

    Keywords

    • ACETATE
    • AMINO ACIDS
    • GLUCOSE
    • GROWTH MEDIUM
    • MORPHOLINEPROPANESULFONIC ACID BUFFER
    • VITAMINS
    • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] gram-positive cocci Lactococcus lactis
    • ACETATE 71-50-1
    • GLUCOSE 50-99-7Q, 58367-01-4Q
    • 10060, Biochemistry studies - General
    • 10063, Biochemistry studies - Vitamins
    • 10064, Biochemistry studies - Proteins, peptides and amino acids
    • 10068, Biochemistry studies - Carbohydrates
    • 31000, Physiology and biochemistry of bacteria
    • 32000, Microbiological apparatus, methods and media
    • Biochemistry and Molecular Biophysics
    • Methods and Techniques
    • Physiology
    • BACTERIA
    • LACTOCOCCUS
    • MEDIA
    • MICROBIOLOGICAL TECHNIQUES
    • Food sciences

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