Abstract
A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h-1. With 19 amino acids added, the growth rate increased to mu = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
| Original language | English |
|---|---|
| Journal | Applied and Environmental Microbiology |
| Volume | 59 |
| Issue number | 12 |
| Pages (from-to) | 4363-4366 |
| Number of pages | 4 |
| ISSN | 0099-2240 |
| DOIs | |
| Publication status | Published - 1993 |
Keywords
- ACETATE
- AMINO ACIDS
- GLUCOSE
- GROWTH MEDIUM
- MORPHOLINEPROPANESULFONIC ACID BUFFER
- VITAMINS
- Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] gram-positive cocci Lactococcus lactis
- ACETATE 71-50-1
- GLUCOSE 50-99-7Q, 58367-01-4Q
- 10060, Biochemistry studies - General
- 10063, Biochemistry studies - Vitamins
- 10064, Biochemistry studies - Proteins, peptides and amino acids
- 10068, Biochemistry studies - Carbohydrates
- 31000, Physiology and biochemistry of bacteria
- 32000, Microbiological apparatus, methods and media
- Biochemistry and Molecular Biophysics
- Methods and Techniques
- Physiology
- BACTERIA
- LACTOCOCCUS
- MEDIA
- MICROBIOLOGICAL TECHNIQUES
- Food sciences