Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins

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    Abstract

    The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P-A1/04/03, or from the growth-rate-dependent Escherichia coli promoter P-rrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.
    Original languageEnglish
    JournalEnvironmental Microbiology
    Volume6
    Pages (from-to)726-732
    ISSN1462-2912
    DOIs
    Publication statusPublished - 2004

    Cite this

    @article{052140c26b084809a8447c477ad006a3,
    title = "Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins",
    abstract = "The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P-A1/04/03, or from the growth-rate-dependent Escherichia coli promoter P-rrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.",
    author = "L. Lambertsen and Claus Sternberg and S{\o}ren Molin",
    year = "2004",
    doi = "10.1111/j.1462-2920.2004.00605.x",
    language = "English",
    volume = "6",
    pages = "726--732",
    journal = "Environmental Microbiology",
    issn = "1462-2912",
    publisher = "Wiley-Blackwell",

    }

    Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins. / Lambertsen, L.; Sternberg, Claus; Molin, Søren.

    In: Environmental Microbiology, Vol. 6, 2004, p. 726-732.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins

    AU - Lambertsen, L.

    AU - Sternberg, Claus

    AU - Molin, Søren

    PY - 2004

    Y1 - 2004

    N2 - The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P-A1/04/03, or from the growth-rate-dependent Escherichia coli promoter P-rrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.

    AB - The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P-A1/04/03, or from the growth-rate-dependent Escherichia coli promoter P-rrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.

    U2 - 10.1111/j.1462-2920.2004.00605.x

    DO - 10.1111/j.1462-2920.2004.00605.x

    M3 - Journal article

    VL - 6

    SP - 726

    EP - 732

    JO - Environmental Microbiology

    JF - Environmental Microbiology

    SN - 1462-2912

    ER -