Mini-chromosomes among danish Candida glabrata isolates originated through two different mechanisms

K. M. Ahmad, O. Ishchuk, L. Hellborg, G. Jørgensen, M. Skvarc, Jesper Stenderup, Dorte Jørck-Ramberg, S. Polakova, Jure Piskur

    Research output: Contribution to journalConference abstract in journalResearchpeer-review


    We analyzed 201 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985 – 1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained mini-chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, C. glabrata strains had independent origin and the analyzed mini-chromosomes were struc-
    turally not related to each other (i.e., they contained different sets o genes). We inferred two mechanisms involved in their origin: (i) through a segmental duplication which covered the centromeric region, and (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of mini-chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in various genetic configurations, including appearance of mini-chromosomes. Such new combinations could have increased fitness in a certain patient ‘‘environment’’
    Original languageEnglish
    Pages (from-to)159-159
    Publication statusPublished - 2012



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