Mimicking Seawater For Culturing Marine Bacteria

Anita Mac Rygaard, Eva Sonnenschein, Lone Gram, Mariane Schmidt Thøgersen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    Only about 1% of marine bacteria have been brought into culture using traditional techniques. The purpose of this study was to investigate if mimicking the natural bacterial environment can increase culturability.We used marine substrates containing defined algal polymers or gellan gum as solidifying agents, and enumerated bacteria from seawater and algal exudates. We tested if culturability could be influenced by addition of quorum sensing signals (AHLs). All plates were incubated at 15°C. Bacterial counts (CFU/g) from algal exudates from brown algae were highest on media containing algal polymers. In general, bacteria isolated from algal exudates preferred more rich media than bacteria isolated from seawater. Overall, culturability ranged from 0.01 to 0.8% as compared to total cell count. Substitution of agar with gellan gum increased the culturability of seawater bacteria approximately 100-fold; from 8.5 x 101 CFU/ml to 5.2 x 103 CFU/ml, whereas addition of AHLs did not improve culturability on any of the media.The substitution of agar with gellan gum shows great promise for increasing culturability of marine bacteria, and further studies are ongoing. The AHLs used in this study were selected based on a previous study determining the most common AHLs produced by marine strains of the Vibrionaceae family. However, their effect on culturability could not be fully explained, so also here further studies are being carried out.
    Original languageEnglish
    Title of host publicationThe Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts
    Place of PublicationCopenhagen
    Publication date2015
    Pages63-63
    Publication statusPublished - 2015
    EventThe Danish Microbiological Society Annual Congress 2015 - Eigtved's Pakhus, Copenhagen, Denmark
    Duration: 9 Nov 20159 Nov 2015

    Conference

    ConferenceThe Danish Microbiological Society Annual Congress 2015
    LocationEigtved's Pakhus
    CountryDenmark
    CityCopenhagen
    Period09/11/201509/11/2015

    Cite this

    Rygaard, A. M., Sonnenschein, E., Gram, L., & Schmidt Thøgersen, M. (2015). Mimicking Seawater For Culturing Marine Bacteria. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts (pp. 63-63). Copenhagen.
    Rygaard, Anita Mac ; Sonnenschein, Eva ; Gram, Lone ; Schmidt Thøgersen, Mariane. / Mimicking Seawater For Culturing Marine Bacteria. The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, 2015. pp. 63-63
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    abstract = "Only about 1{\%} of marine bacteria have been brought into culture using traditional techniques. The purpose of this study was to investigate if mimicking the natural bacterial environment can increase culturability.We used marine substrates containing defined algal polymers or gellan gum as solidifying agents, and enumerated bacteria from seawater and algal exudates. We tested if culturability could be influenced by addition of quorum sensing signals (AHLs). All plates were incubated at 15°C. Bacterial counts (CFU/g) from algal exudates from brown algae were highest on media containing algal polymers. In general, bacteria isolated from algal exudates preferred more rich media than bacteria isolated from seawater. Overall, culturability ranged from 0.01 to 0.8{\%} as compared to total cell count. Substitution of agar with gellan gum increased the culturability of seawater bacteria approximately 100-fold; from 8.5 x 101 CFU/ml to 5.2 x 103 CFU/ml, whereas addition of AHLs did not improve culturability on any of the media.The substitution of agar with gellan gum shows great promise for increasing culturability of marine bacteria, and further studies are ongoing. The AHLs used in this study were selected based on a previous study determining the most common AHLs produced by marine strains of the Vibrionaceae family. However, their effect on culturability could not be fully explained, so also here further studies are being carried out.",
    author = "Rygaard, {Anita Mac} and Eva Sonnenschein and Lone Gram and {Schmidt Th{\o}gersen}, Mariane",
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    Rygaard, AM, Sonnenschein, E, Gram, L & Schmidt Thøgersen, M 2015, Mimicking Seawater For Culturing Marine Bacteria. in The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, pp. 63-63, The Danish Microbiological Society Annual Congress 2015, Copenhagen, Denmark, 09/11/2015.

    Mimicking Seawater For Culturing Marine Bacteria. / Rygaard, Anita Mac; Sonnenschein, Eva; Gram, Lone; Schmidt Thøgersen, Mariane.

    The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, 2015. p. 63-63.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    TY - ABST

    T1 - Mimicking Seawater For Culturing Marine Bacteria

    AU - Rygaard, Anita Mac

    AU - Sonnenschein, Eva

    AU - Gram, Lone

    AU - Schmidt Thøgersen, Mariane

    PY - 2015

    Y1 - 2015

    N2 - Only about 1% of marine bacteria have been brought into culture using traditional techniques. The purpose of this study was to investigate if mimicking the natural bacterial environment can increase culturability.We used marine substrates containing defined algal polymers or gellan gum as solidifying agents, and enumerated bacteria from seawater and algal exudates. We tested if culturability could be influenced by addition of quorum sensing signals (AHLs). All plates were incubated at 15°C. Bacterial counts (CFU/g) from algal exudates from brown algae were highest on media containing algal polymers. In general, bacteria isolated from algal exudates preferred more rich media than bacteria isolated from seawater. Overall, culturability ranged from 0.01 to 0.8% as compared to total cell count. Substitution of agar with gellan gum increased the culturability of seawater bacteria approximately 100-fold; from 8.5 x 101 CFU/ml to 5.2 x 103 CFU/ml, whereas addition of AHLs did not improve culturability on any of the media.The substitution of agar with gellan gum shows great promise for increasing culturability of marine bacteria, and further studies are ongoing. The AHLs used in this study were selected based on a previous study determining the most common AHLs produced by marine strains of the Vibrionaceae family. However, their effect on culturability could not be fully explained, so also here further studies are being carried out.

    AB - Only about 1% of marine bacteria have been brought into culture using traditional techniques. The purpose of this study was to investigate if mimicking the natural bacterial environment can increase culturability.We used marine substrates containing defined algal polymers or gellan gum as solidifying agents, and enumerated bacteria from seawater and algal exudates. We tested if culturability could be influenced by addition of quorum sensing signals (AHLs). All plates were incubated at 15°C. Bacterial counts (CFU/g) from algal exudates from brown algae were highest on media containing algal polymers. In general, bacteria isolated from algal exudates preferred more rich media than bacteria isolated from seawater. Overall, culturability ranged from 0.01 to 0.8% as compared to total cell count. Substitution of agar with gellan gum increased the culturability of seawater bacteria approximately 100-fold; from 8.5 x 101 CFU/ml to 5.2 x 103 CFU/ml, whereas addition of AHLs did not improve culturability on any of the media.The substitution of agar with gellan gum shows great promise for increasing culturability of marine bacteria, and further studies are ongoing. The AHLs used in this study were selected based on a previous study determining the most common AHLs produced by marine strains of the Vibrionaceae family. However, their effect on culturability could not be fully explained, so also here further studies are being carried out.

    M3 - Conference abstract in proceedings

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    EP - 63

    BT - The Danish Microbiological Society Annual Congress 2015

    CY - Copenhagen

    ER -

    Rygaard AM, Sonnenschein E, Gram L, Schmidt Thøgersen M. Mimicking Seawater For Culturing Marine Bacteria. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen. 2015. p. 63-63