Milk-derived GM(3) and GD(3) differentially inhibit dendritic cell maturation and effector functionalities

H. Bronnum, T. Seested, Lars Hellgren, Susanne Brix Pedersen, H. Frokiaer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides in breast milk has yet to be elucidated but when milk is ingested, dietary gangliosides might conceptually affect immune cells, such as dendritic cells (DCs). In this study, we address the in vitro effect of GD(3) and GM(3) on DC effector functionalities. Treatment of bone marrow-derived DCs with GD(3) before lipopolysaccharide-induced maturation decreased the production of interleukin-6 (IL-6), IL-10, IL-12 and tumor necrosis factor-alpha as well as reduced the alloreactivity in mixed leucocyte reaction (MLR). In contrast, only IL-10 and IL-12 productions were significantly inhibited by GM(3,) and the potency of DCs to activate CD4(+) cells in MLR was unaffected by GM(3). However, both gangliosides suppressed expression of CD40, CD80, CD86 and major histocompatibility complex class II on DCs. Because GD(3) overall inhibits DC functionalities more than GM(3), the immune modulating effect of the ganglioside fraction of breast milk might be more prominent in the commencement of lactation during which the milk contains the most GD(3).
    Original languageEnglish
    JournalScandinavian Journal of Immunology
    Volume61
    Issue number6
    Pages (from-to)551-557
    ISSN0300-9475
    DOIs
    Publication statusPublished - 2005

    Cite this

    @article{288b480a7d2c4a2a80f7215845cea1bd,
    title = "Milk-derived GM(3) and GD(3) differentially inhibit dendritic cell maturation and effector functionalities",
    abstract = "Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides in breast milk has yet to be elucidated but when milk is ingested, dietary gangliosides might conceptually affect immune cells, such as dendritic cells (DCs). In this study, we address the in vitro effect of GD(3) and GM(3) on DC effector functionalities. Treatment of bone marrow-derived DCs with GD(3) before lipopolysaccharide-induced maturation decreased the production of interleukin-6 (IL-6), IL-10, IL-12 and tumor necrosis factor-alpha as well as reduced the alloreactivity in mixed leucocyte reaction (MLR). In contrast, only IL-10 and IL-12 productions were significantly inhibited by GM(3,) and the potency of DCs to activate CD4(+) cells in MLR was unaffected by GM(3). However, both gangliosides suppressed expression of CD40, CD80, CD86 and major histocompatibility complex class II on DCs. Because GD(3) overall inhibits DC functionalities more than GM(3), the immune modulating effect of the ganglioside fraction of breast milk might be more prominent in the commencement of lactation during which the milk contains the most GD(3).",
    author = "H. Bronnum and T. Seested and Lars Hellgren and Pedersen, {Susanne Brix} and H. Frokiaer",
    year = "2005",
    doi = "10.1111/j.1365-3083.2005.01566.x",
    language = "English",
    volume = "61",
    pages = "551--557",
    journal = "Scandinavian Journal of Immunology",
    issn = "0300-9475",
    publisher = "Wiley-Blackwell",
    number = "6",

    }

    Milk-derived GM(3) and GD(3) differentially inhibit dendritic cell maturation and effector functionalities. / Bronnum, H.; Seested, T.; Hellgren, Lars; Pedersen, Susanne Brix; Frokiaer, H.

    In: Scandinavian Journal of Immunology, Vol. 61, No. 6, 2005, p. 551-557.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Milk-derived GM(3) and GD(3) differentially inhibit dendritic cell maturation and effector functionalities

    AU - Bronnum, H.

    AU - Seested, T.

    AU - Hellgren, Lars

    AU - Pedersen, Susanne Brix

    AU - Frokiaer, H.

    PY - 2005

    Y1 - 2005

    N2 - Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides in breast milk has yet to be elucidated but when milk is ingested, dietary gangliosides might conceptually affect immune cells, such as dendritic cells (DCs). In this study, we address the in vitro effect of GD(3) and GM(3) on DC effector functionalities. Treatment of bone marrow-derived DCs with GD(3) before lipopolysaccharide-induced maturation decreased the production of interleukin-6 (IL-6), IL-10, IL-12 and tumor necrosis factor-alpha as well as reduced the alloreactivity in mixed leucocyte reaction (MLR). In contrast, only IL-10 and IL-12 productions were significantly inhibited by GM(3,) and the potency of DCs to activate CD4(+) cells in MLR was unaffected by GM(3). However, both gangliosides suppressed expression of CD40, CD80, CD86 and major histocompatibility complex class II on DCs. Because GD(3) overall inhibits DC functionalities more than GM(3), the immune modulating effect of the ganglioside fraction of breast milk might be more prominent in the commencement of lactation during which the milk contains the most GD(3).

    AB - Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides in breast milk has yet to be elucidated but when milk is ingested, dietary gangliosides might conceptually affect immune cells, such as dendritic cells (DCs). In this study, we address the in vitro effect of GD(3) and GM(3) on DC effector functionalities. Treatment of bone marrow-derived DCs with GD(3) before lipopolysaccharide-induced maturation decreased the production of interleukin-6 (IL-6), IL-10, IL-12 and tumor necrosis factor-alpha as well as reduced the alloreactivity in mixed leucocyte reaction (MLR). In contrast, only IL-10 and IL-12 productions were significantly inhibited by GM(3,) and the potency of DCs to activate CD4(+) cells in MLR was unaffected by GM(3). However, both gangliosides suppressed expression of CD40, CD80, CD86 and major histocompatibility complex class II on DCs. Because GD(3) overall inhibits DC functionalities more than GM(3), the immune modulating effect of the ganglioside fraction of breast milk might be more prominent in the commencement of lactation during which the milk contains the most GD(3).

    U2 - 10.1111/j.1365-3083.2005.01566.x

    DO - 10.1111/j.1365-3083.2005.01566.x

    M3 - Journal article

    VL - 61

    SP - 551

    EP - 557

    JO - Scandinavian Journal of Immunology

    JF - Scandinavian Journal of Immunology

    SN - 0300-9475

    IS - 6

    ER -