Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies

D. Tornehave, D.M. Hougaard, L.I. Larsson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Many methods have been devised for double immunocytochemical staining. We now describe that moderate microwaving does not elute antibodies, but prevents their reactions with subsequently applied reagents. Thus, microwaving performed in between the first and second staining cycles permits double indirect immunofluorescence staining with antibodies raised in the same species. Moreover, microwaving also inhibits reactions with endogenous immunoglobulins present in extracellular compartments. This substantially reduces background in indirect immunostaining of mouse tissues with mouse monoclonal antibodies.
    Original languageEnglish
    JournalHistochemistry and Cell Biology
    Volume113
    Issue number1
    Pages (from-to)19-23
    ISSN0948-6143
    DOIs
    Publication statusPublished - 2000

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