microRNA regulation in rainbow trout infected with a fish pathogenic rhabdovirus

Brian Dall Schyth, Seyed Amir Hossein Jalali Hajiabadi, Lasse Bøgelund Juel Kristensen, Finn Skou Pedersen, Niels Lorenzen

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    Abstract

    Rainbow trout is a major worldwide aquaculture species and viral disease has a high cost to fish farmers every year why effective treatment and a deeper understanding of immune components involved in the coexistence between fish and virus is of big concern to our field. We present here a study of microRNA regulation in rainbow trout during infection with the fish pathogenic rhabdovirus viral haemorrhagic septicaemia virus (VHSV). Infected fish as well as infected and immune stimulated cell cultures have been tested for microRNA regulation by microarray using a ´all species´ approach followed by qPCR. Two regulated rainbow trout microRNAs have been cloned, sequenced and upstream promoter areas characterized and tested for functionality upon immune stimulation.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    EventKeystone Symposium on Mechanisms and Biology of Silencing - Monterey, California, USA
    Duration: 1 Jan 2011 → …

    Conference

    ConferenceKeystone Symposium on Mechanisms and Biology of Silencing
    CityMonterey, California, USA
    Period01/01/2011 → …

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