Recombinant microbial cells and methods for producing 5HTP, melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5- hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O- methyltransferase; and means for providing tetrahydrobiopterin (THB), and can be further genetically modified to enrich one or more of tryptophan, S-adenosyl-L-methinonine and acetyl coenzyme A. Related sequences and vectors for use in preparing such recombinant microbial cells are also described.
|IPC||C12P 17/ 10 A I|
|Country||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 12 Mar 2015|