Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system

Filippo Bosco, J. Yang, C. H. Chen, E. Hwu, Stephan Sylvest Keller, Michael Bache, Q. Lin, Anja Boisen

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    A plug-and-play CD-like platform is used to perform a statistical detection of Platelet Derived Growth Factor (PDGF) proteins through aptamer-based surface functionalization of microcantilevers. When PDGF proteins bind to the aptamers, the cantilevers deflect. This deflection is monitored by optical readout heads from a DVD-ROM. The improved sensing platform facilitates measurements in continuous liquid flow with temperature control. Also, the wobbling of the CD platform has been reduced to a minimum and the scanning system has been optimized in order to detect cantilever deflections in liquid in the nanometer range. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We have performed statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.
    Original languageEnglish
    Title of host publicationProceedings of IEEE micro electro mechanical systems
    Publication date2012
    Pages858 - 861
    ISBN (Print)9781467303248
    DOIs
    Publication statusPublished - 2012
    Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems - Paris Marriott Rive Bauche Hotel and Conference Center, Paris, France
    Duration: 29 Jan 20122 Feb 2012
    Conference number: 25th

    Conference

    Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems
    Number25th
    LocationParis Marriott Rive Bauche Hotel and Conference Center
    CountryFrance
    CityParis
    Period29/01/201202/02/2012

    Keywords

    • biosensors
    • cantilevers
    • digital versatile discs
    • micromechanical devices
    • proteins
    • statistical analysis

    Cite this

    Bosco, F., Yang, J., Chen, C. H., Hwu, E., Keller, S. S., Bache, M., ... Boisen, A. (2012). Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system. In Proceedings of IEEE micro electro mechanical systems (pp. 858 - 861 ) https://doi.org/10.1109/MEMSYS.2012.6170321
    Bosco, Filippo ; Yang, J. ; Chen, C. H. ; Hwu, E. ; Keller, Stephan Sylvest ; Bache, Michael ; Lin, Q. ; Boisen, Anja. / Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system. Proceedings of IEEE micro electro mechanical systems. 2012. pp. 858 - 861
    @inproceedings{f2ae0b17d2b544e7b65dcf77c343c48a,
    title = "Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system",
    abstract = "A plug-and-play CD-like platform is used to perform a statistical detection of Platelet Derived Growth Factor (PDGF) proteins through aptamer-based surface functionalization of microcantilevers. When PDGF proteins bind to the aptamers, the cantilevers deflect. This deflection is monitored by optical readout heads from a DVD-ROM. The improved sensing platform facilitates measurements in continuous liquid flow with temperature control. Also, the wobbling of the CD platform has been reduced to a minimum and the scanning system has been optimized in order to detect cantilever deflections in liquid in the nanometer range. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We have performed statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.",
    keywords = "biosensors, cantilevers, digital versatile discs, micromechanical devices, proteins, statistical analysis",
    author = "Filippo Bosco and J. Yang and Chen, {C. H.} and E. Hwu and Keller, {Stephan Sylvest} and Michael Bache and Q. Lin and Anja Boisen",
    year = "2012",
    doi = "10.1109/MEMSYS.2012.6170321",
    language = "English",
    isbn = "9781467303248",
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    booktitle = "Proceedings of IEEE micro electro mechanical systems",

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    Bosco, F, Yang, J, Chen, CH, Hwu, E, Keller, SS, Bache, M, Lin, Q & Boisen, A 2012, Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system. in Proceedings of IEEE micro electro mechanical systems. pp. 858 - 861 , 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, Paris, France, 29/01/2012. https://doi.org/10.1109/MEMSYS.2012.6170321

    Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system. / Bosco, Filippo; Yang, J.; Chen, C. H.; Hwu, E.; Keller, Stephan Sylvest; Bache, Michael; Lin, Q.; Boisen, Anja.

    Proceedings of IEEE micro electro mechanical systems. 2012. p. 858 - 861 .

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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    AU - Keller, Stephan Sylvest

    AU - Bache, Michael

    AU - Lin, Q.

    AU - Boisen, Anja

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    AB - A plug-and-play CD-like platform is used to perform a statistical detection of Platelet Derived Growth Factor (PDGF) proteins through aptamer-based surface functionalization of microcantilevers. When PDGF proteins bind to the aptamers, the cantilevers deflect. This deflection is monitored by optical readout heads from a DVD-ROM. The improved sensing platform facilitates measurements in continuous liquid flow with temperature control. Also, the wobbling of the CD platform has been reduced to a minimum and the scanning system has been optimized in order to detect cantilever deflections in liquid in the nanometer range. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We have performed statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.

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    Bosco F, Yang J, Chen CH, Hwu E, Keller SS, Bache M et al. Micromechanical aptasensor-based protein detection using a compact-disc format microfluidics system. In Proceedings of IEEE micro electro mechanical systems. 2012. p. 858 - 861 https://doi.org/10.1109/MEMSYS.2012.6170321