Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer

Emil Laust Kristoffersen, Morten Leth Jepsen, Birgitta R. Knudsen, Yi-Ping Ho

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Cells are defined as the basic functional unit of living organisms. They sense environmental stimuli, respond and adapt to the environmental changes chemically or physically in order to form and maintain the distinct tissues in complex biological organisms. Therefore, even cells of identical genetic identities can display a wide disparity of physical responses (such as cell morphologies) or chemical responses (such as RNA or protein expression level regulation). These asynchronous responses to the microenvironments, often due to random fl uctuations, or noise in gene especially when techniques with single cell sensitivity are not available. Hence, currently our understanding of cells are in many aspects limited by the inherent ensemble average obtained by analyzing bulk populations of cells, which does not necessary precisely represent the individual behavior of each cell within a population.
Original languageEnglish
Title of host publicationNanomedicine
EditorsKenneth A. Howard, Thomas Vorup-Jensen, Dan Peer
PublisherSpringer
Publication date2016
Pages77-95
Chapter5
ISBN (Print)978-1-4939-3632-8
ISBN (Electronic) 978-1-4939-3634-2
DOIs
Publication statusPublished - 2016
Externally publishedYes
SeriesAdvances in Delivery Science and Technology
ISSN2192-6212

Keywords

  • Nanosensors
  • Circulating tumor cells
  • Genomic analysis
  • Enzymatic activities

Cite this

Kristoffersen, E. L., Jepsen, M. L., Knudsen, B. R., & Ho, Y-P. (2016). Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer. In K. A. Howard, T. Vorup-Jensen, & D. Peer (Eds.), Nanomedicine (pp. 77-95). Springer. Advances in Delivery Science and Technology https://doi.org/10.1007/978-1-4939-3634-2_5
Kristoffersen, Emil Laust ; Jepsen, Morten Leth ; Knudsen, Birgitta R. ; Ho, Yi-Ping. / Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer. Nanomedicine. editor / Kenneth A. Howard ; Thomas Vorup-Jensen ; Dan Peer. Springer, 2016. pp. 77-95 (Advances in Delivery Science and Technology).
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abstract = "Cells are defined as the basic functional unit of living organisms. They sense environmental stimuli, respond and adapt to the environmental changes chemically or physically in order to form and maintain the distinct tissues in complex biological organisms. Therefore, even cells of identical genetic identities can display a wide disparity of physical responses (such as cell morphologies) or chemical responses (such as RNA or protein expression level regulation). These asynchronous responses to the microenvironments, often due to random fl uctuations, or noise in gene especially when techniques with single cell sensitivity are not available. Hence, currently our understanding of cells are in many aspects limited by the inherent ensemble average obtained by analyzing bulk populations of cells, which does not necessary precisely represent the individual behavior of each cell within a population.",
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Kristoffersen, EL, Jepsen, ML, Knudsen, BR & Ho, Y-P 2016, Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer. in KA Howard, T Vorup-Jensen & D Peer (eds), Nanomedicine. Springer, Advances in Delivery Science and Technology, pp. 77-95. https://doi.org/10.1007/978-1-4939-3634-2_5

Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer. / Kristoffersen, Emil Laust; Jepsen, Morten Leth; Knudsen, Birgitta R.; Ho, Yi-Ping.

Nanomedicine. ed. / Kenneth A. Howard; Thomas Vorup-Jensen; Dan Peer. Springer, 2016. p. 77-95 (Advances in Delivery Science and Technology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

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N2 - Cells are defined as the basic functional unit of living organisms. They sense environmental stimuli, respond and adapt to the environmental changes chemically or physically in order to form and maintain the distinct tissues in complex biological organisms. Therefore, even cells of identical genetic identities can display a wide disparity of physical responses (such as cell morphologies) or chemical responses (such as RNA or protein expression level regulation). These asynchronous responses to the microenvironments, often due to random fl uctuations, or noise in gene especially when techniques with single cell sensitivity are not available. Hence, currently our understanding of cells are in many aspects limited by the inherent ensemble average obtained by analyzing bulk populations of cells, which does not necessary precisely represent the individual behavior of each cell within a population.

AB - Cells are defined as the basic functional unit of living organisms. They sense environmental stimuli, respond and adapt to the environmental changes chemically or physically in order to form and maintain the distinct tissues in complex biological organisms. Therefore, even cells of identical genetic identities can display a wide disparity of physical responses (such as cell morphologies) or chemical responses (such as RNA or protein expression level regulation). These asynchronous responses to the microenvironments, often due to random fl uctuations, or noise in gene especially when techniques with single cell sensitivity are not available. Hence, currently our understanding of cells are in many aspects limited by the inherent ensemble average obtained by analyzing bulk populations of cells, which does not necessary precisely represent the individual behavior of each cell within a population.

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Kristoffersen EL, Jepsen ML, Knudsen BR, Ho Y-P. Microfluidics-based Single Cell Analytical Platforms for Characterization of Cancer. In Howard KA, Vorup-Jensen T, Peer D, editors, Nanomedicine. Springer. 2016. p. 77-95. (Advances in Delivery Science and Technology). https://doi.org/10.1007/978-1-4939-3634-2_5