Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

Krishna Kant, Mohammad-Ali Shahbazi, Vivek Priy Dave, Tien Anh Ngo, Vinayaka Aaydha Chidambara, Quyen Than Linh, Dang Duong Bang, Anders Wolff*

*Corresponding author for this work

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Abstract

Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Novel strategies mainly rely on incorporating all the steps from sample preparation to detection in miniaturized devices for online monitoring of pathogens with high accuracy and sensitivity in a time-saving and cost effective manner. Lab on chip is a blooming area in diagnosis, which exploits different mechanical and biological techniques to detect very low concentrations of pathogens in food samples. This is achieved through streamlining the sample handling and concentrating procedures, which will subsequently reduce human errors and enhance the accuracy of the sensing methods. Integration of sample preparation techniques into these devices can effectively minimize the impact of complex food matrix on pathogen diagnosis and improve the limit of detections. Integration of pathogen capturing bio-receptors on microfluidic devices is a crucial step, which can facilitate recognition abilities in harsh chemical and physical conditions, offering a great commercial benefit to the food-manufacturing sector. This article reviews recent advances in current state-of-the-art of sample preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line.
Original languageEnglish
JournalBiotechnology Advances
Volume36
Issue number4
Pages (from-to)1003-1024
Number of pages22
ISSN0734-9750
DOIs
Publication statusPublished - 2018

Keywords

  • Acoustophoresis
  • DNA amplification
  • Electrochemical biosensor
  • Foodborne pathogen
  • Immunological detection
  • Lab-on-a-chip
  • Magnetophoresis
  • Microfluidic device
  • Optical biosensor
  • Point of care detection

Cite this

Kant, Krishna ; Shahbazi, Mohammad-Ali ; Dave, Vivek Priy ; Ngo, Tien Anh ; Aaydha Chidambara, Vinayaka ; Than Linh, Quyen ; Bang, Dang Duong ; Wolff, Anders. / Microfluidic devices for sample preparation and rapid detection of foodborne pathogens. In: Biotechnology Advances. 2018 ; Vol. 36, No. 4. pp. 1003-1024.
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abstract = "Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Novel strategies mainly rely on incorporating all the steps from sample preparation to detection in miniaturized devices for online monitoring of pathogens with high accuracy and sensitivity in a time-saving and cost effective manner. Lab on chip is a blooming area in diagnosis, which exploits different mechanical and biological techniques to detect very low concentrations of pathogens in food samples. This is achieved through streamlining the sample handling and concentrating procedures, which will subsequently reduce human errors and enhance the accuracy of the sensing methods. Integration of sample preparation techniques into these devices can effectively minimize the impact of complex food matrix on pathogen diagnosis and improve the limit of detections. Integration of pathogen capturing bio-receptors on microfluidic devices is a crucial step, which can facilitate recognition abilities in harsh chemical and physical conditions, offering a great commercial benefit to the food-manufacturing sector. This article reviews recent advances in current state-of-the-art of sample preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line.",
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Microfluidic devices for sample preparation and rapid detection of foodborne pathogens. / Kant, Krishna; Shahbazi, Mohammad-Ali; Dave, Vivek Priy; Ngo, Tien Anh; Aaydha Chidambara, Vinayaka; Than Linh, Quyen; Bang, Dang Duong; Wolff, Anders.

In: Biotechnology Advances, Vol. 36, No. 4, 2018, p. 1003-1024.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

AU - Kant, Krishna

AU - Shahbazi, Mohammad-Ali

AU - Dave, Vivek Priy

AU - Ngo, Tien Anh

AU - Aaydha Chidambara, Vinayaka

AU - Than Linh, Quyen

AU - Bang, Dang Duong

AU - Wolff, Anders

PY - 2018

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KW - Acoustophoresis

KW - DNA amplification

KW - Electrochemical biosensor

KW - Foodborne pathogen

KW - Immunological detection

KW - Lab-on-a-chip

KW - Magnetophoresis

KW - Microfluidic device

KW - Optical biosensor

KW - Point of care detection

U2 - 10.1016/j.biotechadv.2018.03.002

DO - 10.1016/j.biotechadv.2018.03.002

M3 - Journal article

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EP - 1024

JO - Biotechnology Advances

JF - Biotechnology Advances

SN - 0734-9750

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ER -