Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Gerardo Perozziello; Patrizio Candeloro; Antonio De Grazia; Francesco Esposito; Marco Allione; Maria Laura Coluccio; Rossana Tallerico; Immanuel Valpapuram; Luca Tirinato; Gobind Das; Andrea Giugni; Bruno Torre; Pierangelo Veltri; Ulrich Krühne; Giuseppe Della Valle; Enzo Di Fabrizio

doi:10.1364/OE.24.00A180

Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Gerardo Perozziello, Patrizio Candeloro, Antonio De Grazia, Francesco Esposito, Marco Allione, Maria Laura Coluccio, Rossana Tallerico, Immanuel Valpapuram, Luca Tirinato, Gobind Das, Andrea Giugni, Bruno Torre, Pierangelo Veltri, Ulrich Krühne, Giuseppe Della Valle, Enzo Di Fabrizio

Department of Chemical and Biochemical Engineering

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Abstract

In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels-where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).

Original language	English
Journal	Optics Express
Volume	24
Issue number	2
Pages (from-to)	A180-A190
Number of pages	11
ISSN	1094-4087
DOIs	https://doi.org/10.1364/OE.24.00A180
Publication status	Published - 2016

Keywords

Integrated optics
Cell analysis

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Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimersFinal published version, 4.25 MB

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Perozziello, G., Candeloro, P., De Grazia, A., Esposito, F., Allione, M., Coluccio, M. L., Tallerico, R., Valpapuram, I., Tirinato, L., Das, G., Giugni, A., Torre, B., Veltri, P., Krühne, U., Della Valle, G., & Di Fabrizio, E. (2016). Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express, 24(2), A180-A190. https://doi.org/10.1364/OE.24.00A180

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title = "Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers",

abstract = "In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels-where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).",

keywords = "Integrated optics, Cell analysis",

author = "Gerardo Perozziello and Patrizio Candeloro and {De Grazia}, Antonio and Francesco Esposito and Marco Allione and Coluccio, {Maria Laura} and Rossana Tallerico and Immanuel Valpapuram and Luca Tirinato and Gobind Das and Andrea Giugni and Bruno Torre and Pierangelo Veltri and Ulrich Kr{\"u}hne and {Della Valle}, Giuseppe and {Di Fabrizio}, Enzo",

year = "2016",

doi = "10.1364/OE.24.00A180",

language = "English",

volume = "24",

pages = "A180--A190",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

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Perozziello, G, Candeloro, P, De Grazia, A, Esposito, F, Allione, M, Coluccio, ML, Tallerico, R, Valpapuram, I, Tirinato, L, Das, G, Giugni, A, Torre, B, Veltri, P, Krühne, U, Della Valle, G & Di Fabrizio, E 2016, 'Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers', Optics Express, vol. 24, no. 2, pp. A180-A190. https://doi.org/10.1364/OE.24.00A180

TY - JOUR

T1 - Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

AU - Perozziello, Gerardo

AU - Candeloro, Patrizio

AU - De Grazia, Antonio

AU - Esposito, Francesco

AU - Allione, Marco

AU - Coluccio, Maria Laura

AU - Tallerico, Rossana

AU - Valpapuram, Immanuel

AU - Tirinato, Luca

AU - Das, Gobind

AU - Giugni, Andrea

AU - Torre, Bruno

AU - Veltri, Pierangelo

AU - Krühne, Ulrich

AU - Della Valle, Giuseppe

AU - Di Fabrizio, Enzo

PY - 2016

Y1 - 2016

N2 - In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels-where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).

AB - In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels-where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).

KW - Integrated optics

KW - Cell analysis

U2 - 10.1364/OE.24.00A180

DO - 10.1364/OE.24.00A180

M3 - Journal article

SN - 1094-4087

VL - 24

SP - A180-A190

JO - Optics Express

JF - Optics Express

IS - 2

ER -

Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

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