Microfluidic device and method for processing of macromolecules

Anders Kristensen (Inventor), Rodolphe Marie (Inventor), Kristian Hagsted Rasmussen (Inventor), Mir Kalim Ullah (Inventor)

    Research output: Patent

    Abstract

    A microfluidic device and method for enzymatic processing of ultra-long macromolecules is disclosed. The device comprises a reaction chamber with a first manifold, a second manifold, and a plurality of reaction channels, each reaction channel extending from the first manifold to the second manifold. The device further comprises first inlet and outlet channels for filling the reaction channels via the manifolds with one or more macromolecule containers suspended in a first carrier fluid, wherein the first inlet and outlet channels are configured such that a flow established from the first set of inlets to the first set of outlets is guided through the reaction channels, and second inlet and outlet channels for feeding an enzymatic reagent to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels, wherein the second set of inlets and outlets are configured such that a flow established from the second inlet to the second outlet is guided through at least one of the manifolds and bypasses the reaction channels.
    Original languageEnglish
    IPCB01L3/00
    Patent numberWO2012055415
    Filing date03/05/2012
    CountryDenmark
    Publication statusPublished - 2012

    Bibliographical note

    DTU reference nummer: 92542-10

    Cite this

    Kristensen, A., Marie, R., Rasmussen, K. H., & Kalim Ullah, M. (2012). IPC No. B01L3/00. Microfluidic device and method for processing of macromolecules. (Patent No. WO2012055415).
    @misc{db88404ad71e47cba10a6618c711e77a,
    title = "Microfluidic device and method for processing of macromolecules",
    abstract = "A microfluidic device and method for enzymatic processing of ultra-long macromolecules is disclosed. The device comprises a reaction chamber with a first manifold, a second manifold, and a plurality of reaction channels, each reaction channel extending from the first manifold to the second manifold. The device further comprises first inlet and outlet channels for filling the reaction channels via the manifolds with one or more macromolecule containers suspended in a first carrier fluid, wherein the first inlet and outlet channels are configured such that a flow established from the first set of inlets to the first set of outlets is guided through the reaction channels, and second inlet and outlet channels for feeding an enzymatic reagent to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels, wherein the second set of inlets and outlets are configured such that a flow established from the second inlet to the second outlet is guided through at least one of the manifolds and bypasses the reaction channels.",
    author = "Anders Kristensen and Rodolphe Marie and Rasmussen, {Kristian Hagsted} and {Kalim Ullah}, Mir",
    note = "DTU reference nummer: 92542-10; WO2012055415; B01L3/00",
    year = "2012",
    language = "English",
    type = "Patent",

    }

    Kristensen, A, Marie, R, Rasmussen, KH & Kalim Ullah, M May. 03 2012, Microfluidic device and method for processing of macromolecules, Patent No. WO2012055415, IPC No. B01L3/00.

    Microfluidic device and method for processing of macromolecules. / Kristensen, Anders (Inventor); Marie, Rodolphe (Inventor); Rasmussen, Kristian Hagsted (Inventor); Kalim Ullah, Mir (Inventor).

    IPC No.: B01L3/00. Patent No.: WO2012055415. May 03, 2012.

    Research output: Patent

    TY - PAT

    T1 - Microfluidic device and method for processing of macromolecules

    AU - Kristensen, Anders

    AU - Marie, Rodolphe

    AU - Rasmussen, Kristian Hagsted

    AU - Kalim Ullah, Mir

    N1 - DTU reference nummer: 92542-10

    PY - 2012

    Y1 - 2012

    N2 - A microfluidic device and method for enzymatic processing of ultra-long macromolecules is disclosed. The device comprises a reaction chamber with a first manifold, a second manifold, and a plurality of reaction channels, each reaction channel extending from the first manifold to the second manifold. The device further comprises first inlet and outlet channels for filling the reaction channels via the manifolds with one or more macromolecule containers suspended in a first carrier fluid, wherein the first inlet and outlet channels are configured such that a flow established from the first set of inlets to the first set of outlets is guided through the reaction channels, and second inlet and outlet channels for feeding an enzymatic reagent to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels, wherein the second set of inlets and outlets are configured such that a flow established from the second inlet to the second outlet is guided through at least one of the manifolds and bypasses the reaction channels.

    AB - A microfluidic device and method for enzymatic processing of ultra-long macromolecules is disclosed. The device comprises a reaction chamber with a first manifold, a second manifold, and a plurality of reaction channels, each reaction channel extending from the first manifold to the second manifold. The device further comprises first inlet and outlet channels for filling the reaction channels via the manifolds with one or more macromolecule containers suspended in a first carrier fluid, wherein the first inlet and outlet channels are configured such that a flow established from the first set of inlets to the first set of outlets is guided through the reaction channels, and second inlet and outlet channels for feeding an enzymatic reagent to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels, wherein the second set of inlets and outlets are configured such that a flow established from the second inlet to the second outlet is guided through at least one of the manifolds and bypasses the reaction channels.

    M3 - Patent

    M1 - WO2012055415

    Y2 - 2012/05/03

    ER -