Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment

Jamil El-Ali; Ivan Ryberg Perch-Nielsen; Claus Riber Poulsen; Malene Jensen; Pieter Telleman; Anders Wolff

doi:10.1109/SENSOR.2003.1215291

Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment

Jamil El-Ali, Ivan Ryberg Perch-Nielsen, Claus Riber Poulsen, Malene Jensen, Pieter Telleman, Anders Wolff

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

We present the first example of a microfabricated PCR thermocycling chip integrated with advanced sample pre-treatment. The device consists of a SU-8 based PCR thermocycler chip monolithically integrated with dielectrophoretic (DEP) sample pre-treatment. This allows on chip purification of samples by removing inhibitors that would otherwise prevent PCR amplification. Integrated thin film heaters and a resistive temperature sensor control the PCR thermocycling. The sample pre-treatment system uses a combination of fluid flow and cell capturing by DEP to perform the sample treatment. The sample pre-treatment system was tested on a sample containing yeast cells and heparin that inhibits PCR. Only treated sample could be successfully amplified using PCR.

Original language	English
Title of host publication	Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)
Place of Publication	Boston, USA
Publication date	2003
Pages	214-217
Article number	1215291
DOIs	https://doi.org/10.1109/SENSOR.2003.1215291
Publication status	Published - 2003
Event	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: 8 Jun 2003 → 12 Jun 2003 Conference number: 12

Conference

Conference	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
Number	12
Country/Territory	United States
City	Boston
Period	08/06/2003 → 12/06/2003

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El-Ali, J., Perch-Nielsen, I. R., Poulsen, C. R., Jensen, M., Telleman, P., & Wolff, A. (2003). Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment. In Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) (pp. 214-217). Article 1215291. https://doi.org/10.1109/SENSOR.2003.1215291

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title = "Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment",

abstract = "We present the first example of a microfabricated PCR thermocycling chip integrated with advanced sample pre-treatment. The device consists of a SU-8 based PCR thermocycler chip monolithically integrated with dielectrophoretic (DEP) sample pre-treatment. This allows on chip purification of samples by removing inhibitors that would otherwise prevent PCR amplification. Integrated thin film heaters and a resistive temperature sensor control the PCR thermocycling. The sample pre-treatment system uses a combination of fluid flow and cell capturing by DEP to perform the sample treatment. The sample pre-treatment system was tested on a sample containing yeast cells and heparin that inhibits PCR. Only treated sample could be successfully amplified using PCR. ",

author = "Jamil El-Ali and Perch-Nielsen, \{Ivan Ryberg\} and Poulsen, \{Claus Riber\} and Malene Jensen and Pieter Telleman and Anders Wolff",

year = "2003",

doi = "10.1109/SENSOR.2003.1215291",

language = "English",

pages = "214--217",

booktitle = "Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)",

note = "12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers ; Conference date: 08-06-2003 Through 12-06-2003",

}

El-Ali, J, Perch-Nielsen, IR, Poulsen, CR, Jensen, M, Telleman, P & Wolff, A 2003, Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment. in Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)., 1215291, Boston, USA, pp. 214-217, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 08/06/2003. https://doi.org/10.1109/SENSOR.2003.1215291

Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment. / El-Ali, Jamil; Perch-Nielsen, Ivan Ryberg; Poulsen, Claus Riber et al.
Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers). Boston, USA, 2003. p. 214-217 1215291.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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AU - Telleman, Pieter

AU - Wolff, Anders

N1 - Conference code: 12

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AB - We present the first example of a microfabricated PCR thermocycling chip integrated with advanced sample pre-treatment. The device consists of a SU-8 based PCR thermocycler chip monolithically integrated with dielectrophoretic (DEP) sample pre-treatment. This allows on chip purification of samples by removing inhibitors that would otherwise prevent PCR amplification. Integrated thin film heaters and a resistive temperature sensor control the PCR thermocycling. The sample pre-treatment system uses a combination of fluid flow and cell capturing by DEP to perform the sample treatment. The sample pre-treatment system was tested on a sample containing yeast cells and heparin that inhibits PCR. Only treated sample could be successfully amplified using PCR.

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DO - 10.1109/SENSOR.2003.1215291

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CY - Boston, USA

T2 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers

Y2 - 8 June 2003 through 12 June 2003

ER -

Microfabricated DNA amplification device monolithically integrated with advanced sample pre-treatment

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