Microencapsulation in genipin cross-linked gelatine-maltodextrin improves survival of Bifidobacterium adolescentis during exposure to in vitro gastrointestinal conditions.

Antonela D. Borza, Nana T. Annan, Debra L. Moreau, Paula M. Allan-Wojtas, Amyl Ghanem, Derick Rousseau, Allan T. Paulson, Lisbeth Truelstrup Hansen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To improve survival during exposure to adverse conditions, probiotic Bifidobacterium adolescentis 15703T cells were encapsulated in novel mono-core and multi-core phase-separated gelatine-maltodextrin (GMD) microspheres where the gelatine (G) phase was cross-linked with genipin (GP). Microscopy showed that encapsulated cells were exclusively associated with maltodextrin (MD) core(s). Small (average diameter 37 pm) and large (70 mu m) GMD and G microspheres were produced by modulating factors (e.g. mixing speed, surfactant, GP and G concentrations) affecting the size, structural stability and phase-separation. In vitro sequential gastro-intestinal (GI) juice challenge experiments revealed increased survival of cells encapsulated in GMD (similar to 10(6-7) cfu mL(-1)) and G (similar to 10(5) cfu mL-1) microspheres as compared to free cells (similar to 10(4) cfu mL-1). In GMD microspheres, the bacteria derive energy from MD to survive during exposure to acid and bile salts. In conclusion, the novel food grade GMD microencapsulation formulation was shown to protect probiotic bifidobacteria from adverse conditions.
Original languageEnglish
JournalJournal of Microencapsulation
Volume27
Issue number5
Pages (from-to)387-399
ISSN0265-2048
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Pharmaceutical Science
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Bioengineering
  • Organic Chemistry
  • Bifidobacteria
  • Gelatine
  • Genipin
  • Maltodextrin
  • Microencapsulation
  • Phase-separation
  • Probiotics
  • bile salt
  • gelatin
  • genipin
  • maltodextrin
  • microsphere
  • probiotic agent
  • article
  • bacterial survival
  • Bifidobacterium adolescentis
  • cell viability
  • controlled study
  • drug delivery system
  • drug dosage form comparison
  • gastrointestinal secretion
  • in vitro study
  • microencapsulation
  • microscopy
  • molecular stability
  • nonhuman
  • particle size
  • phase separation
  • stomach juice
  • structure activity relation
  • Bifidobacterium
  • Cells, Immobilized
  • Cross-Linking Reagents
  • Drug Compounding
  • Gastric Juice
  • Gastrointestinal Tract
  • Gelatin
  • Iridoid Glycosides
  • Phase Transition
  • Polysaccharides
  • Iridoids
  • 6902-77-8 genipin
  • 9000-70-8 Gelatin
  • 9050-36-6 maltodextrin
  • CHEMISTRY,
  • ENGINEERING,
  • PHARMACOLOGY
  • LACTIC-ACID BACTERIA
  • PHASE-SEPARATION
  • PROBIOTIC BACTERIA
  • GELATIN/MALTODEXTRIN MIXTURES
  • FRUCTO-OLIGOSACCHARIDES
  • LINKING REACTION
  • GASTRIC-JUICE
  • LOW-PH
  • BILE
  • MICROSPHERES
  • bifidobacteria
  • gelatine
  • phase-separation
  • gastrointestinal condition
  • species survival
  • structural stability
  • Actinomycetes and Related Organisms Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Irregular Nonsporing Gram-Positive Rods [08890] Bifidobacterium adolescentis species strain-15703T
  • Bifidobacterium adolescentis probiotic nutrient
  • bile acid
  • 12512, Pathology - Therapy
  • 13202, Nutrition - General studies, nutritional status and methods
  • 14004, Digestive system - Physiology and biochemistry
  • 22002, Pharmacology - General
  • 31000, Physiology and biochemistry of bacteria
  • Ingestion and Assimilation
  • Pharmacology
  • genipin cross-linked gelatine-maltodextrin microencapsulation laboratory techniques
  • microscopy laboratory techniques, imaging and microscopy techniques
  • Digestive System
  • Nutrition
  • Pharmaceuticals
  • MICROENCAPSULATION

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