Microchip Coulter particle counter

Ulrik Darling Larsen; Gert Blankenstein; J. Branebjerg

doi:10.1109/SENSOR.1997.635479

Microchip Coulter particle counter

Ulrik Darling Larsen, Gert Blankenstein, J. Branebjerg

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during operation. By sheathing of a nonconductive liquid on either side of an electrolyte, it is possible to optimize the sensitivity to a specific cell-size. A method for measuring the relative flow-rates between liquid phases with different conductivity is presented. The method utilizes the laminar flow and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding

Original language	English
Title of host publication	Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Volume	2
Publisher	IEEE
Publication date	1997
Pages	1319-1322
ISBN (Print)	0-7803-3829-4
DOIs	https://doi.org/10.1109/SENSOR.1997.635479
Publication status	Published - 1997
Event	International Conference on Solid-state Sensors and Actuators (Transducers 1997) - Chicago,IL, United States Duration: 16 Jun 1997 → 19 Jun 1997

Conference

Conference	International Conference on Solid-state Sensors and Actuators (Transducers 1997)
Country/Territory	United States
City	Chicago,IL
Period	16/06/1997 → 19/06/1997

Bibliographical note

Copyright: 1997 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE

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title = "Microchip Coulter particle counter",

abstract = "This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during operation. By sheathing of a nonconductive liquid on either side of an electrolyte, it is possible to optimize the sensitivity to a specific cell-size. A method for measuring the relative flow-rates between liquid phases with different conductivity is presented. The method utilizes the laminar flow and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding",

author = "Larsen, {Ulrik Darling} and Gert Blankenstein and J. Branebjerg",

note = "Copyright: 1997 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE; International Conference on Solid-state Sensors and Actuators (Transducers 1997), Transducers '97 ; Conference date: 16-06-1997 Through 19-06-1997",

year = "1997",

doi = "10.1109/SENSOR.1997.635479",

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booktitle = "Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on",

publisher = "IEEE",

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Larsen, UD, Blankenstein, G & Branebjerg, J 1997, Microchip Coulter particle counter. in Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on. vol. 2, IEEE, pp. 1319-1322, International Conference on Solid-state Sensors and Actuators (Transducers 1997), Chicago,IL, United States, 16/06/1997. https://doi.org/10.1109/SENSOR.1997.635479

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AU - Larsen, Ulrik Darling

AU - Blankenstein, Gert

AU - Branebjerg, J.

N1 - Copyright: 1997 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE

PY - 1997

Y1 - 1997

N2 - This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during operation. By sheathing of a nonconductive liquid on either side of an electrolyte, it is possible to optimize the sensitivity to a specific cell-size. A method for measuring the relative flow-rates between liquid phases with different conductivity is presented. The method utilizes the laminar flow and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding

AB - This paper presents a micro device employing the Coulter principle for counting and sizing of living cells and particles in liquid suspension. The microchip Coulter particle counter (μCPC) has been employed in a planar silicon structure covered with glass, which enables detailed observation during operation. By sheathing of a nonconductive liquid on either side of an electrolyte, it is possible to optimize the sensitivity to a specific cell-size. A method for measuring the relative flow-rates between liquid phases with different conductivity is presented. The method utilizes the laminar flow and short contact time of liquids in microchannels. As a result, the width of the liquids can be controlled without knowing the actual flow rates. The μCPC has been fabricated by standard microfabrication techniques, including RIE, wet silicon etching, metalization and anodic bonding

U2 - 10.1109/SENSOR.1997.635479

DO - 10.1109/SENSOR.1997.635479

M3 - Article in proceedings

SN - 0-7803-3829-4

VL - 2

SP - 1319

EP - 1322

BT - Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on

PB - IEEE

T2 - International Conference on Solid-state Sensors and Actuators (Transducers 1997)

Y2 - 16 June 1997 through 19 June 1997

ER -

Microchip Coulter particle counter

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