Microbiota composition of simultaneously colonized mice housed under either a gnotobiotic isolator or individually ventilated cage regime

Randi Lundberg, Martin Iain Bahl, Tine Rask Licht, Martin F. Toft, Axel K. Hansen

Research output: Contribution to journalJournal articleResearchpeer-review

526 Downloads (Pure)

Abstract

Germ-free rodents colonized with microbiotas of interest are used for host-microbiota investigations and for testing microbiota-targeted therapeutic candidates. Traditionally, isolators are used for housing such gnotobiotic rodents due to optimal protection from the environment, but research groups focused on the microbiome are increasingly combining or substituting isolator housing with individually ventilated cage (IVC) systems. We compared the effect of housing systems on the gut microbiota composition of germ-free mice colonized with a complex microbiota and housed in either multiple IVC cages in an IVC facility or in multiple open-top cages in an isolator during three generations and five months. No increase in bacterial diversity as assessed by 16S rRNA gene sequencing was observed in the IVC cages, despite not applying completely aseptic cage changes. The donor bacterial community was equally represented in both housing systems. Time-dependent clustering between generations was observed in both systems, but was strongest in the IVC cages. Different relative abundance of a Rikenellaceae genus contributed to separate clustering of the isolator and IVC communities. Our data suggest that complex microbiotas are protected in IVC systems, but challenges related to temporal dynamics should be addressed.
Original languageEnglish
Article number42245
JournalScientific Reports
Volume7
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2017

Fingerprint

Dive into the research topics of 'Microbiota composition of simultaneously colonized mice housed under either a gnotobiotic isolator or individually ventilated cage regime'. Together they form a unique fingerprint.

Cite this