Projects per year
Violations of temperature regulations in the meat chain may affect meat safety. Methods are lacking to estimate whether meat has been subjected to temperature abuse. Exposure to too high temperatures may lead to systematic changes in the diverse bacterial communities of fresh meat. We investigated whether temperature induced changes in the community composition on fresh meat surfaces can reflect the temperature-history (combination of time and temperature). Sterile pieces of pork were inoculated with a carcass swab homogenate, to which Salmonella was added. Changes in the meat microbiota were monitored during aerobic chill-storage (4 °C and 7 °C) and temperature abuse (12 °C and 16 °C) for 96 hours, by culture-based methods and 16S rRNA gene sequencing. Bacterial genera that dominated during prolonged temperature abuse were Acinetobacter, Serratia and Pseudomonas, whereas chill-stored meat was dominated by Pseudomonas only. We also showed that the initial community affects subsequent changes during storage. The results suggest that principal coordinate analysis of beta diversity could be a useful tool to reveal temperature abused meat. Sequence data and culturing data revealed a strong positive association between growth of Escherichia coli and growth of Salmonella, which suggests that Escherichia coli can be used as indicator of temperature-history supporting growth of Salmonella on fresh pork surfaces.
|Title of host publication||The Danish Microbiological Society Annual Congress 2017 - Programme & Abstracts|
|Place of Publication||Copenhagen, Denmark|
|Publisher||American Society for Microbiology|
|Publication status||Published - 2017|
|Event||Danish Microbiological Society 2017 Congress - Eigtveds Pakhus, Copenhagne, Denmark|
Duration: 13 Nov 2017 → 13 Nov 2017
|Conference||Danish Microbiological Society 2017 Congress|
|Period||13/11/2017 → 13/11/2017|
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- 1 Finished
Meat-Cross-Con: Meat-Cross-Con: Meat safety - An innovative modelling approach to evaluate microbial pathogen transfer and cross contamination from farm to fork
Aabo, S., Bahl, M. I., Nauta, M., Møller, C. O. D. A., Buschhardt, T., Sørensen, S. J., Gombossy de Melo Franco, B. D., Sant’Ana, A. D. S., da Silva, A. P. R., Lopes, J. & Hansen, T. B.
Danish Council for Strategic Research
01/01/2013 → 31/12/2015