Microbial community response to ammonia levels in hydrogen assisted biogas production and upgrading process

Han Wang, Xinyu Zhu, Qun Yan, Yifeng Zhang*, Irini Angelidaki

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1-7 g NH4+-N L-1). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.
Original languageEnglish
Article number122276
JournalBioresource Technology
Volume296
Number of pages7
ISSN0960-8524
DOIs
Publication statusPublished - 2020

Keywords

  • Anaerobic digestion
  • Biogas upgrading
  • Microbial community
  • Ammonia inhibition
  • Hydrogenotrophic methanogens

Cite this

@article{165a781b2a564a59822087d90eadf341,
title = "Microbial community response to ammonia levels in hydrogen assisted biogas production and upgrading process",
abstract = "Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1-7 g NH4+-N L-1). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.",
keywords = "Anaerobic digestion, Biogas upgrading, Microbial community, Ammonia inhibition, Hydrogenotrophic methanogens",
author = "Han Wang and Xinyu Zhu and Qun Yan and Yifeng Zhang and Irini Angelidaki",
year = "2020",
doi = "10.1016/j.biortech.2019.122276",
language = "English",
volume = "296",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Microbial community response to ammonia levels in hydrogen assisted biogas production and upgrading process. / Wang, Han; Zhu, Xinyu; Yan, Qun; Zhang, Yifeng; Angelidaki, Irini.

In: Bioresource Technology, Vol. 296, 122276, 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Microbial community response to ammonia levels in hydrogen assisted biogas production and upgrading process

AU - Wang, Han

AU - Zhu, Xinyu

AU - Yan, Qun

AU - Zhang, Yifeng

AU - Angelidaki, Irini

PY - 2020

Y1 - 2020

N2 - Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1-7 g NH4+-N L-1). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.

AB - Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1-7 g NH4+-N L-1). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.

KW - Anaerobic digestion

KW - Biogas upgrading

KW - Microbial community

KW - Ammonia inhibition

KW - Hydrogenotrophic methanogens

U2 - 10.1016/j.biortech.2019.122276

DO - 10.1016/j.biortech.2019.122276

M3 - Journal article

VL - 296

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 122276

ER -