Microbial Community Evolution and Fate of Antibiotic Resistance Genes during Sludge Treatment in Two Full-Scale Anaerobic Digestion Plants with Thermal Hydrolysis Pretreatment

Juan Tong, Ping Fang, Junya Zhang, Yuansong Wei*, Yanyan Su, Yifeng Zhang

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Anaerobic digestion (AD) with thermal hydrolysis pretreatment is widely used as an efficient sludge treatment nowadays. However, the evolution of microbial community (especially for the archaea community), the fate of antibiotic resistance genes (ARGs), and their associations during such process in full-scale sludge treatment plants are rarely reported. Therefore, these scientific questions were explored at two full-scale sludge treatment plants through high-throughput sequencing and quantitative PCR. Results showed that Methanobacterium and Methanosphaera were the dominant archaea in thermal hydrolyzed sludge. The predominant bacteria in the sludge first shifted from nutrients removal functional bacteria to spore-forming bacteria after thermal hydrolysis, and then shifted to fermentative bacteria after AD. The full-scale plants could select ermB, ermF, mefA/E, qnrS and tetM. Though the bacteria and archaea biomass and community largely influenced the fate of ARGs, multiple linear regression analysis showed that the total ARGs were mainly affected by mobile genetic elements (MGEs).
Original languageEnglish
Article number121575
JournalBioresource Technology
Volume288
Number of pages10
ISSN0960-8524
DOIs
Publication statusPublished - 2019

Keywords

  • Sludge thermal hydrolysis
  • Anaerobic digestion
  • Microbial community
  • Antibitotic resistance genes
  • Multiple linear regression analysis

Fingerprint Dive into the research topics of 'Microbial Community Evolution and Fate of Antibiotic Resistance Genes during Sludge Treatment in Two Full-Scale Anaerobic Digestion Plants with Thermal Hydrolysis Pretreatment'. Together they form a unique fingerprint.

Cite this