Microbial cells and methods for production of hernandulcin

Irina Borodina; Jonathan Asmund Arnesen; Jane Dannow Dyekjær

Microbial cells and methods for production of hernandulcin

Irina Borodina (Inventor), Jonathan Asmund Arnesen (Inventor), Jane Dannow Dyekjær (Inventor)

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Abstract

The present invention relates to microbial production of the sweet non-calorigenic sesquiterpenoid hernandulcin. Disclosed herein are yeast cells capable of producing hernandulcin and optionally hernandulcin derivatives, said yeast cells expressing at least one (+)-epi-alpha-bisabolol synthase, at least one cytochrome P450 enzyme (CYP) and at least one cytochrome P450 reductase, preferably said CYP the Nicotiana tabacum CYP 5-epi-aristolocene dihydroxylase (NtEAH) or the Datura stramonium cytochrome P450 enzyme DsEAH.

Original language	English
IPC	C12P 5/ 00 A I
Patent number	WO2023242403
Filing date	16/06/2022
Country/Territory	International Bureau of the World Intellectual Property Organization (WIPO)
Priority date	16/06/2022
Priority number	EP20220179430
Publication status	Published - 21 Dec 2023

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title = "Microbial cells and methods for production of hernandulcin",

abstract = "The present invention relates to microbial production of the sweet non-calorigenic sesquiterpenoid hernandulcin. Disclosed herein are yeast cells capable of producing hernandulcin and optionally hernandulcin derivatives, said yeast cells expressing at least one (+)-epi-alpha-bisabolol synthase, at least one cytochrome P450 enzyme (CYP) and at least one cytochrome P450 reductase, preferably said CYP the Nicotiana tabacum CYP 5-epi-aristolocene dihydroxylase (NtEAH) or the Datura stramonium cytochrome P450 enzyme DsEAH.",

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T1 - Microbial cells and methods for production of hernandulcin

AU - Borodina, Irina

AU - Arnesen, Jonathan Asmund

AU - Dyekjær, Jane Dannow

PY - 2023/12/21

Y1 - 2023/12/21

N2 - The present invention relates to microbial production of the sweet non-calorigenic sesquiterpenoid hernandulcin. Disclosed herein are yeast cells capable of producing hernandulcin and optionally hernandulcin derivatives, said yeast cells expressing at least one (+)-epi-alpha-bisabolol synthase, at least one cytochrome P450 enzyme (CYP) and at least one cytochrome P450 reductase, preferably said CYP the Nicotiana tabacum CYP 5-epi-aristolocene dihydroxylase (NtEAH) or the Datura stramonium cytochrome P450 enzyme DsEAH.

AB - The present invention relates to microbial production of the sweet non-calorigenic sesquiterpenoid hernandulcin. Disclosed herein are yeast cells capable of producing hernandulcin and optionally hernandulcin derivatives, said yeast cells expressing at least one (+)-epi-alpha-bisabolol synthase, at least one cytochrome P450 enzyme (CYP) and at least one cytochrome P450 reductase, preferably said CYP the Nicotiana tabacum CYP 5-epi-aristolocene dihydroxylase (NtEAH) or the Datura stramonium cytochrome P450 enzyme DsEAH.

M3 - Patent

M1 - WO2023242403

Y2 - 2022/06/16

ER -

Microbial cells and methods for production of hernandulcin

Abstract

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