Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications

Salim Bouaidat, C. Berendsen, P. Thomsen, S.G. Petersen, Anders Wolff, J. Jonsmann

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Micro scale patterning of bioactive surfaces is desirable for numerous biochip applications. Polyethyleneoxide-like (PEO-like) coating with non-fouling functionality has been deposited using low frequency AC plasma polymerization. The non-fouling properties of the coating were tested with human cells ( HeLa) and fluorescence labeled proteins (isothiocyanate-labeled bovine serum albumin, i.e. FITC-BSA). The PEO-like coatings were fabricated by plasma polymerization of 12-crown-4 (ppCrown) with plasma polymerized hexene (ppHexene) as adhesion layer. The coatings were micro patterned using conventional cleanroom photolithography and lift-off. Single cell arrays showed sharp contrast in cell adhesion between the untreated glass surface and the ppCrown layer. Similarly, proteins adsorbed selectively to untreated glass but not to ppCrown. The simplicity of the liftoff technique and the sturdiness and versatility of the plasma-polymerized coatings, make this technology highly suitable for bio-MEMS and biochip applications, where patterned high contrast non-fouling surfaces are needed.
    Original languageEnglish
    JournalLab on a Chip
    Volume4
    Issue number6
    Pages (from-to)632-637
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2004

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