Abstract
The invention relates to a genetically modified lactic acid bacterium capable of producing (S,S)-2,3-butanediol stereo specifically from glucose under aerobic conditions. Additionally the invention relates to a method for producing (S,S)-2,3-butanediol and L-acetoin using the genetically modified lactic acid bacterium, under aerobic conditions in the presence of a source of iron-containing porphyrin or a source of metal ions (Fe3+/Fe2+). The lactic acid bacterium is genetically modified to express heterologous genes encoding enzymes catalysing the stereo-specific synthesis of (S,S)-2,3-butanediol; and additionally a number of genes are deleted in order to maximise the production of (S,S)-2,3-butanediol as compared to other products of oxidative fermentation.
| Original language | English |
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| IPC | C12R 1/ 225 A N |
| Patent number | WO2016097268 |
| Filing date | 23/06/2016 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 27/10/2015 |
| Priority number | EP20150191703 |
| Publication status | Published - 23 Jun 2016 |