Micro-droplet based directed evolution outperforms conventional laboratory evolution

Staffan L. Sjostrom; Mingtao Huang; Jens Nielsen; Haakan N. Joensson; Helene Andersson Svahn

Micro-droplet based directed evolution outperforms conventional laboratory evolution

Staffan L. Sjostrom, Mingtao Huang, Jens Nielsen, Haakan N. Joensson, Helene Andersson Svahn

Novo Nordisk Foundation Center for Biosustainability

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Abstract

We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.

Original language	English
Journal	International Conference on Miniaterized Systems for Chemistry and Life Sciences (proceedings)
Pages (from-to)	169-171
Number of pages	3
ISSN	1556-5890
Publication status	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 Conference number: 18 https://microtasconferences.org/microtas2014/

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	18
Country/Territory	United States
City	San Antonio
Period	26/10/2014 → 30/10/2014
Internet address	https://microtasconferences.org/microtas2014/

Keywords

Directed evolution
Droplet microfluidics
High throughput
Enzymes
Cell factories

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title = "Micro-droplet based directed evolution outperforms conventional laboratory evolution",

abstract = "We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.",

keywords = "Directed evolution, Droplet microfluidics, High throughput, Enzymes, Cell factories",

author = "Sjostrom, {Staffan L.} and Mingtao Huang and Jens Nielsen and Joensson, {Haakan N.} and Svahn, {Helene Andersson}",

year = "2014",

language = "English",

pages = "169--171",

journal = "International Conference on Miniaterized Systems for Chemistry and Life Sciences (proceedings)",

issn = "1556-5890",

publisher = "Transducers Research Foundations",

note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

url = "https://microtasconferences.org/microtas2014/",

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TY - ABST

T1 - Micro-droplet based directed evolution outperforms conventional laboratory evolution

AU - Sjostrom, Staffan L.

AU - Huang, Mingtao

AU - Nielsen, Jens

AU - Joensson, Haakan N.

AU - Svahn, Helene Andersson

N1 - Conference code: 18

PY - 2014

Y1 - 2014

N2 - We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.

AB - We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.

KW - Directed evolution

KW - Droplet microfluidics

KW - High throughput

KW - Enzymes

KW - Cell factories

M3 - Conference abstract in journal

SN - 1556-5890

SP - 169

EP - 171

JO - International Conference on Miniaterized Systems for Chemistry and Life Sciences (proceedings)

JF - International Conference on Miniaterized Systems for Chemistry and Life Sciences (proceedings)

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 26 October 2014 through 30 October 2014

ER -

Micro-droplet based directed evolution outperforms conventional laboratory evolution

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