Micro-droplet based directed evolution outperforms conventional laboratory evolution

Staffan L. Sjostrom, Mingtao Huang, Jens Nielsen, Haakan N. Joensson, Helene Andersson Svahn

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Abstract

We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.
Original languageEnglish
JournalInternational Conference on Miniaterized Systems for Chemistry and Life Sciences (proceedings)
Pages (from-to)169-171
Number of pages3
ISSN1556-5890
Publication statusPublished - 2014
EventThe 18th International Conference on Miniaterized Systems for Chemistry and Life Sciences: MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014
Conference number: 18

Conference

ConferenceThe 18th International Conference on Miniaterized Systems for Chemistry and Life Sciences
Number18
CountryUnited States
CitySan Antonio
Period26/10/201430/10/2014

Keywords

  • Directed evolution
  • Droplet microfluidics
  • High throughput
  • Enzymes
  • Cell factories

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