Micro and nano-platforms for biological cell analysis

Winnie Edith Svendsen, Jaime Castillo, Jacob Lange Moresco, Luigi Sasso, Mark Holm Olsen, Mohammed Jassim Ismaiil Al-Abaddi, Lars Andresen, Simon Levinsen, Pranjul Jaykumar Shah, Indumathi Vedarethinam, Maria Dimaki

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In this paper some technological platforms developed for biological cell analysis will be presented and compared to existing systems. In brief, we present a novel micro cell culture chamber based on diffusion feeding of cells, into which cells can be introduced and extracted after culturing using normal pipettes, thus making it readily usable for clinical laboratories. To enhance the functionality of such a chamber we have been investigating the use of active or passive 3D surface modifications. Active modifications involve miniature electrodes able to record electrical or electrochemical signals from the cells, while passive modifications involve the presence of a peptide nanotube based scaffold for the cell culturing that mimics the in vivo environment. Two applications involving fluorescent in situ hybridization (FISH) analysis and cancer cell sorting are presented, as examples of further analysis that can be done after cell culturing. A platform able to automate the entire process from cell culturing to cell analysis by means of simple plug and play of various self-contained, individually fabricated modules is finally described.
    Original languageEnglish
    JournalSensors and Actuators A: Physical
    Volume172
    Issue number1
    Pages (from-to)54-60
    ISSN0924-4247
    DOIs
    Publication statusPublished - 2011
    EventEurosensors XXIV - Linz, Austria
    Duration: 1 Jan 2010 → …

    Conference

    ConferenceEurosensors XXIV
    CityLinz, Austria
    Period01/01/2010 → …

    Keywords

    • Cell sorting
    • Nanoelectrodes
    • Cell culturing
    • Peptide nanotubes

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