MHC-based detection of antigen-specific CD8(+) T cell responses

Sine Reker Hadrup, Ton N. Schumacher

Research output: Contribution to journalReviewResearchpeer-review

Abstract

The hallmark of adaptive immunity is its ability to recognise a wide range of antigens and technologies that capture this diversity are therefore of substantial interest. New methods have recently been developed that allow the parallel analysis of T cell reactivity against vast numbers of different epitopes in limited biological material. These technologies are based on the joint binding of differentially labelled MHC multimers on the T cell surface, thereby providing each antigen-specific T cell population with a unique multicolour code. This strategy of 'combinatorial encoding' enables detection of many (at least 25) different T cell populations per sample and should be of broad value for both T cell epitope identification and immunomonitoring.
Original languageEnglish
JournalCancer Immunology, Immunotherapy
Volume59
Issue number9
Pages (from-to)1425-1433
Number of pages9
ISSN0340-7004
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Cancer Research
  • Oncology
  • Immunology
  • Immunology and Allergy
  • Combinatorial encoding
  • Epitope mapping
  • MHC multimers
  • MHC tetramers
  • Quantum dots
  • T cell detection
  • allophycocyanin
  • HLA A2 antigen
  • HLA A3 antigen
  • phycoerythrin
  • quantum dot
  • adaptive immunity
  • antigen specificity
  • CD8+ T lymphocyte
  • cell selection
  • cross reaction
  • cytokine release
  • DNA microarray
  • enzyme linked immunosorbent assay
  • epitope mapping
  • flow cytometry
  • fluorochrome staining
  • high throughput screening
  • human
  • major histocompatibility complex
  • priority journal
  • review
  • sensitivity and specificity
  • Animals
  • Antigens
  • CD8-Positive T-Lymphocytes
  • Epitope Mapping
  • Histocompatibility Antigens
  • Humans
  • Monitoring, Immunologic
  • Protein Binding
  • Protein Multimerization
  • T-Lymphocyte Subsets
  • ONCOLOGY
  • IMMUNOLOGY
  • CLASS-I LIGANDS
  • METASTATIC MELANOMA
  • P/MHC MICROARRAYS
  • EPITOPE DISCOVERY
  • PEPTIDE EXCHANGE
  • FLOW-CYTOMETRY
  • IDENTIFICATION
  • TECHNOLOGY
  • RECEPTOR
  • THERAPY
  • Animalia (Animals) - Animalia [33000] animal common
  • antigen
  • MHC major histocompatibility complex
  • 02506, Cytology - Animal
  • 10060, Biochemistry studies - General
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 34502, Immunology - General and methods
  • Chemical Coordination and Homeostasis
  • Transport and Circulation
  • CD8-positive T cell immune system, blood and lymphatics
  • Biochemistry and Molecular Biophysics
  • Blood and Lymphatics
  • Immune System

Fingerprint

Dive into the research topics of 'MHC-based detection of antigen-specific CD8(+) T cell responses'. Together they form a unique fingerprint.

Cite this