Abstract
The hallmark of adaptive immunity is its ability to recognise a wide range of antigens and technologies that capture this diversity are therefore of substantial interest. New methods have recently been developed that allow the parallel analysis of T cell reactivity against vast numbers of different epitopes in limited biological material. These technologies are based on the joint binding of differentially labelled MHC multimers on the T cell surface, thereby providing each antigen-specific T cell population with a unique multicolour code. This strategy of 'combinatorial encoding' enables detection of many (at least 25) different T cell populations per sample and should be of broad value for both T cell epitope identification and immunomonitoring.
| Original language | English |
|---|---|
| Journal | Cancer Immunology, Immunotherapy |
| Volume | 59 |
| Issue number | 9 |
| Pages (from-to) | 1425-1433 |
| Number of pages | 9 |
| ISSN | 0340-7004 |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
Keywords
- Cancer Research
- Oncology
- Immunology
- Immunology and Allergy
- Combinatorial encoding
- Epitope mapping
- MHC multimers
- MHC tetramers
- Quantum dots
- T cell detection
- allophycocyanin
- HLA A2 antigen
- HLA A3 antigen
- phycoerythrin
- quantum dot
- adaptive immunity
- antigen specificity
- CD8+ T lymphocyte
- cell selection
- cross reaction
- cytokine release
- DNA microarray
- enzyme linked immunosorbent assay
- epitope mapping
- flow cytometry
- fluorochrome staining
- high throughput screening
- human
- major histocompatibility complex
- priority journal
- review
- sensitivity and specificity
- Animals
- Antigens
- CD8-Positive T-Lymphocytes
- Epitope Mapping
- Histocompatibility Antigens
- Humans
- Monitoring, Immunologic
- Protein Binding
- Protein Multimerization
- T-Lymphocyte Subsets
- ONCOLOGY
- IMMUNOLOGY
- CLASS-I LIGANDS
- METASTATIC MELANOMA
- P/MHC MICROARRAYS
- EPITOPE DISCOVERY
- PEPTIDE EXCHANGE
- FLOW-CYTOMETRY
- IDENTIFICATION
- TECHNOLOGY
- RECEPTOR
- THERAPY
- Animalia (Animals) - Animalia [33000] animal common
- antigen
- MHC major histocompatibility complex
- 02506, Cytology - Animal
- 10060, Biochemistry studies - General
- 10064, Biochemistry studies - Proteins, peptides and amino acids
- 15002, Blood - Blood and lymph studies
- 15004, Blood - Blood cell studies
- 34502, Immunology - General and methods
- Chemical Coordination and Homeostasis
- Transport and Circulation
- CD8-positive T cell immune system, blood and lymphatics
- Biochemistry and Molecular Biophysics
- Blood and Lymphatics
- Immune System
