Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy.

Tim Tolker-Nielsen, Claus Sternberg

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


    In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.
    Original languageEnglish
    Title of host publicationPseudomonas Methods and Protocols
    EditorsAlain Filloux, Juan-Luis Ramos
    Number of pages15
    Publication date2014
    ISBN (Print)978-1-4939-0472-3
    Publication statusPublished - 2014
    SeriesMethods in Molecular Biology


    Dive into the research topics of 'Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy.'. Together they form a unique fingerprint.

    Cite this