Abstract
| Original language | English |
|---|---|
| Title of host publication | Campylobacter jejuni - methods and protocols |
| Editors | James Butcher, Alain Stintzi |
| Number of pages | 10 |
| Publisher | Springer Science+Business Media |
| Publication date | 2017 |
| Pages | 19-28 |
| Chapter | 3 |
| ISBN (Print) | 978-1-4939-6534-2 |
| ISBN (Electronic) | 978-1-4939-6536-6 |
| Publication status | Published - 2017 |
| Series | Methods in Molecular Biology |
|---|---|
| Number | 1512 |
| ISSN | 1064-3745 |
Keywords
- Bacteriophage
- C. jejuni
- Campylobacter
- Isolation
- Propagation
- Purification
- Titration
Cite this
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Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. / Gencay, Yilmaz Emre; Birk, Tina; Sørensen, Martine Camilla Holst; Brøndsted, Lone.
Campylobacter jejuni - methods and protocols. ed. / James Butcher; Alain Stintzi. Springer Science+Business Media, 2017. p. 19-28 (Methods in Molecular Biology; No. 1512).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
TY - CHAP
T1 - Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni
AU - Gencay, Yilmaz Emre
AU - Birk, Tina
AU - Sørensen, Martine Camilla Holst
AU - Brøndsted, Lone
PY - 2017
Y1 - 2017
N2 - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
AB - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
KW - Bacteriophage
KW - C. jejuni
KW - Campylobacter
KW - Isolation
KW - Propagation
KW - Purification
KW - Titration
M3 - Book chapter
SN - 978-1-4939-6534-2
SP - 19
EP - 28
BT - Campylobacter jejuni - methods and protocols
A2 - Butcher, James
A2 - Stintzi, Alain
PB - Springer Science+Business Media
ER -