Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni

Yilmaz Emre Gencay, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
Original languageEnglish
Title of host publicationCampylobacter jejuni - methods and protocols
EditorsJames Butcher, Alain Stintzi
Number of pages10
PublisherSpringer Science+Business Media
Publication date2017
Pages19-28
Chapter3
ISBN (Print)978-1-4939-6534-2
ISBN (Electronic)978-1-4939-6536-6
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
Number1512
ISSN1064-3745

Keywords

  • Bacteriophage
  • C. jejuni
  • Campylobacter
  • Isolation
  • Propagation
  • Purification
  • Titration

Cite this

Gencay, Y. E., Birk, T., Sørensen, M. C. H., & Brøndsted, L. (2017). Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. In J. Butcher, & A. Stintzi (Eds.), Campylobacter jejuni - methods and protocols (pp. 19-28). Springer Science+Business Media. Methods in Molecular Biology, No. 1512
Gencay, Yilmaz Emre ; Birk, Tina ; Sørensen, Martine Camilla Holst ; Brøndsted, Lone. / Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. Campylobacter jejuni - methods and protocols. editor / James Butcher ; Alain Stintzi. Springer Science+Business Media, 2017. pp. 19-28 (Methods in Molecular Biology; No. 1512).
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Gencay, YE, Birk, T, Sørensen, MCH & Brøndsted, L 2017, Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. in J Butcher & A Stintzi (eds), Campylobacter jejuni - methods and protocols. Springer Science+Business Media, Methods in Molecular Biology, no. 1512, pp. 19-28.

Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. / Gencay, Yilmaz Emre; Birk, Tina; Sørensen, Martine Camilla Holst; Brøndsted, Lone.

Campylobacter jejuni - methods and protocols. ed. / James Butcher; Alain Stintzi. Springer Science+Business Media, 2017. p. 19-28 (Methods in Molecular Biology; No. 1512).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

TY - CHAP

T1 - Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni

AU - Gencay, Yilmaz Emre

AU - Birk, Tina

AU - Sørensen, Martine Camilla Holst

AU - Brøndsted, Lone

PY - 2017

Y1 - 2017

N2 - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

AB - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

KW - Bacteriophage

KW - C. jejuni

KW - Campylobacter

KW - Isolation

KW - Propagation

KW - Purification

KW - Titration

M3 - Book chapter

SN - 978-1-4939-6534-2

SP - 19

EP - 28

BT - Campylobacter jejuni - methods and protocols

A2 - Butcher, James

A2 - Stintzi, Alain

PB - Springer Science+Business Media

ER -

Gencay YE, Birk T, Sørensen MCH, Brøndsted L. Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni. In Butcher J, Stintzi A, editors, Campylobacter jejuni - methods and protocols. Springer Science+Business Media. 2017. p. 19-28. (Methods in Molecular Biology; No. 1512).