Method validation for strobilurin fungicides in cereals and fruit

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Abstract

Strobilurins are a new class of fungicides that are active against a broad spectrum of fungi. In the present work a GC method for analysis of strobilurin fungicides was validated. The method was based on extraction with ethyl acetate/cyclohexane, clean-up by gel permeation chromatography (GPC) and determination of the content by gas chromatography (GC) with electron capture (EC-), nitrogen/phosphorous (NP-), and mass spectrometric (MS-) detection. Three strobilurins, azoxystrobin, kresoxim-methyl and trifloxystrobin were validated on three matrices, wheat, apple and grapes. The validation was based on recovery tests at three or four spiking levels, determined as double determinations and repeated three times (n=6). The mean recoveries for the three detectors were in the range of 70-114%, and the LODs were between 0.004 mg/kg and 0.014 mg/kg, for all three strobilurins. The values for repeatability and reproducibility were within the limits for repeatability and reproducibility given by the Horwitz equation. Validation was not accepted for azoxystrobin in grapes on all three detectors and for azoxystrobin in apple for the MS-detector. A comparison of matrix-matched standards versus standards in solvent showed varying differences between the two calibration curves.
Original languageEnglish
JournalFood Additives and Contaminants
Volume18
Issue number10
Pages (from-to)866-874
ISSN0265-203X
DOIs
Publication statusPublished - 2001
Event3rd European Pesticide Residues Workshop - York, United Kingdom
Duration: 3 Jul 20005 Jul 2000

Workshop

Workshop3rd European Pesticide Residues Workshop
CountryUnited Kingdom
CityYork
Period03/07/200005/07/2000

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