Method for Screening Compounds That Influence Virulence Gene Expression in Staphylococcus aureus

A. Nielsen, Kristian Fog Nielsen, D. Frees, Thomas Ostenfeld Larsen, H. Ingmer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic beta-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
    Original languageEnglish
    JournalAntimicrobial Agents and Chemotherapy
    Volume54
    Issue number1
    Pages (from-to)509-512
    ISSN0066-4804
    DOIs
    Publication statusPublished - 2010

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