Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

Jette Kjeldgaard; Sidsel Henriksen; Marianne Thorup Cohn; Søren Aabo; Hanne Ingmer

doi:10.1128/AEM.05471-11

Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

Jette Kjeldgaard, Sidsel Henriksen, Marianne Thorup Cohn, Søren Aabo, Hanne Ingmer

National Food Institute

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	23
Pages (from-to)	8456-8458
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.05471-11
Publication status	Published - 2011

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title = "Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix",

abstract = "We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.",

author = "Jette Kjeldgaard and Sidsel Henriksen and Cohn, {Marianne Thorup} and S{\o}ren Aabo and Hanne Ingmer",

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T1 - Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

AU - Kjeldgaard, Jette

AU - Henriksen, Sidsel

AU - Cohn, Marianne Thorup

AU - Aabo, Søren

AU - Ingmer, Hanne

PY - 2011

Y1 - 2011

N2 - We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

AB - We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

U2 - 10.1128/AEM.05471-11

DO - 10.1128/AEM.05471-11

M3 - Journal article

SN - 0099-2240

VL - 77

SP - 8456

EP - 8458

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 23

ER -

Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

Abstract

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