Abstract
| Original language | English |
|---|---|
| Journal | Applied Microbiology and Biotechnology |
| Volume | 103 |
| Issue number | 2 |
| Pages (from-to) | 1007-1019 |
| ISSN | 0175-7598 |
| DOIs | |
| Publication status | Published - 2019 |
Keywords
- Drinking water
- Methanotrophs
- Pesticides
- Phenoxy acids
- Removal
Cite this
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Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter. / Papadopoulou, Aikaterini; Hedegaard, Mathilde Jørgensen; Dechesne, Arnaud; Albrechtsen, Hans-Jørgen; Musovic, Sanin; Smets, Barth F.
In: Applied Microbiology and Biotechnology, Vol. 103, No. 2, 2019, p. 1007-1019.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter
AU - Papadopoulou, Aikaterini
AU - Hedegaard, Mathilde Jørgensen
AU - Dechesne, Arnaud
AU - Albrechtsen, Hans-Jørgen
AU - Musovic, Sanin
AU - Smets, Barth F.
PY - 2019
Y1 - 2019
N2 - Drinking water supply is in many parts of the world based on groundwater. Groundwater often contains methane, which can be oxidized by methanotrophs upon aeration. Sand from rapid sand filters fed with methane-rich groundwater can remove some pesticides (Hedegaard and Albrechtsen in Water Res 48:71-81, 2014). We enriched methanotrophs from filter sand and investigated whether they could drive the degradation of various pesticides. To enrich for methanotrophs, we designed and operated four laboratory-scale, continuously methane-fed column reactors, inoculated with filter sand and one control column fed with tap water. When enrichments were obtained, methane was continuously supplied to three reactors, while the fourth was starved for methane for 1 week, and the reactors were spiked with ten pesticides at groundwater-relevant concentrations (2.1-6.6 μg/L). Removal for most pesticides was not detected at the investigated contact time (1.37 min). However, the degradation of phenoxy acids was observed in the methanotrophic column reactor starved for methane, while it was not detected in the control column indicating the importance of methanotrophs. Phenoxy acid removal, using dichlorprop as a model compound, was further investigated in batch experiments with methanotrophic biomass collected from the enrichment reactors. Phenoxy acid removal (expressed per gram of matrix sand) was substantially improved in the methanotrophic enrichment compared to parent filter sand. The presence of methane did not clearly impact dichlorprop removal but did impact mineralization. We suggest that other heterotrophs are responsible for the first step in dichlorprop degradation, while the subsequent steps including ring-hydroxylation are driven by methanotrophs.
AB - Drinking water supply is in many parts of the world based on groundwater. Groundwater often contains methane, which can be oxidized by methanotrophs upon aeration. Sand from rapid sand filters fed with methane-rich groundwater can remove some pesticides (Hedegaard and Albrechtsen in Water Res 48:71-81, 2014). We enriched methanotrophs from filter sand and investigated whether they could drive the degradation of various pesticides. To enrich for methanotrophs, we designed and operated four laboratory-scale, continuously methane-fed column reactors, inoculated with filter sand and one control column fed with tap water. When enrichments were obtained, methane was continuously supplied to three reactors, while the fourth was starved for methane for 1 week, and the reactors were spiked with ten pesticides at groundwater-relevant concentrations (2.1-6.6 μg/L). Removal for most pesticides was not detected at the investigated contact time (1.37 min). However, the degradation of phenoxy acids was observed in the methanotrophic column reactor starved for methane, while it was not detected in the control column indicating the importance of methanotrophs. Phenoxy acid removal, using dichlorprop as a model compound, was further investigated in batch experiments with methanotrophic biomass collected from the enrichment reactors. Phenoxy acid removal (expressed per gram of matrix sand) was substantially improved in the methanotrophic enrichment compared to parent filter sand. The presence of methane did not clearly impact dichlorprop removal but did impact mineralization. We suggest that other heterotrophs are responsible for the first step in dichlorprop degradation, while the subsequent steps including ring-hydroxylation are driven by methanotrophs.
KW - Drinking water
KW - Methanotrophs
KW - Pesticides
KW - Phenoxy acids
KW - Removal
U2 - 10.1007/s00253-018-9501-8
DO - 10.1007/s00253-018-9501-8
M3 - Journal article
C2 - 30474728
VL - 103
SP - 1007
EP - 1019
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -